E06 mAb-biotinylated 330002S-25UG

NACRES:

NA.45

Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).
E06 mAb-biotinylated has been used in immunodetection. It may be used in immunohistochemistry, western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL), oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys, mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.
0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)

antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
assay	>95%
packaging	pkg of 1 x 0.125 mL (polypropylene tube with screw cap (330002S-25ug))
Торговая марка	Avanti Polar Lipids
concentration	0.2 mg/mL (330002S-25ug)
shipped in	dry ice
storage temp.	−70°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable