Lysozyme human

CAS Number:	12671-19-1
Enzyme Commission (EC) Number:	3.2.1.17 ( BRENDA | IUBMB )
MDL number:	MFCD00131557
NACRES:	NA.54

Кат. номер

L1667-10G

L1667-1G

L1667-100MG

L1667-10MG

Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains.
Lysozyme human has been used:

• in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques
• in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system
• in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)

Packaging
1, 10 g in glass bottle
Package size based on protein content
Biochem/physiol Actions
Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.
Features and Benefits
Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:

• Improves Bacterial Fermentation Efficiency
• Greater bacterial lysis activity per mg.
• 1 gram of delivers more lysis activity than 4 grams of HEWL
• Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
• Improved recombinant protein yield compared to mechanical lysis
• Reduces cost of goods

Unit Definition
One unit will produce a A450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Lysobac is a trademark of InVitria

Quality Level	200
recombinant	expressed in rice
assay	90% (SDS-PAGE)
form	lyophilized powder
specific activity	100,000 units/mg protein
mol wt	16.5 kDa
UniProt accession no.	P61626,
shipped in	dry ice
storage temp.	70°C
Gene Information	human ... LYZ(4069)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable