YeastBuster Protein Extraction Reagent

Кат. номер

71186-4REF

71186-EA

71186-4

71186-4CN

71186-3

71186-3CN

Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
YeastBuster<TMSYMBOL></TMSYMBOL> Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with -1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
Packaging
100, 500 ml in Plastic ampoule
Features and Benefits

• Gentle, rapid, efficient extraction of proteins from yeast cells
• Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
• Higher yield of total and enzymatically active proteins
• Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods

Components
•100 ml or 500 mlYeastBuster Protein Extraction Reagent
•1 ml or 5 ml100X THP Solution
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Multiple storage requirements
Торговая марка	Novagen®
storage condition	OK to freeze
shipped in	wet ice

pictograms	GHS08
signalword	Warning
hcodes	H373
pcodes	P260 - P314 - P501
Target Organs	Kidney
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable