Benzonase® Nuclease

CAS Number:	9025-65-4
Enzyme Commission (EC) Number:	3.1.30.2 ( BRENDA | IUBMB )
MDL number:	MFCD00131010
NACRES:	NA.54

Кат. номер

E1014-25KU

E1014-5KU

E1014-5KU-KC

E1014-5KU-PW

E1014-25KU-KC

E1014-25KU-PW

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.
Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorimetric experiments.
Used for the removal of nucleic acid from protein samples.
Benzonase® is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is found to be 8.0-9.2. It completely digests nucleic acids to 5- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids.
Digests native or heat-denatured DNA and RNA.
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a A260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Benzonase® Nuclease is supplied by Merck KGaA, Darmstadt, Germany and/or its affiliates.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
recombinant	expressed in E. coli
assay	90% (SDS-PAGE)
form	buffered aqueous glycerol solution
mol wt	30 kDa
concentration	250 units/µL
foreign activity	protease, essentially free
shipped in	wet ice
storage temp.	20°C

Personal Protective Equipment	Eyeshields, Gloves
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable