Tryptose Phosphate Broth solution

NACRES:

NA.75

Кат. номер

T8159-PH

T8159-100ML

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Quality Level	300
sterility	sterile-filtered
form	solution
concentration	29.5 g/L in deionized water
application(s)	cell culture | mammalian: suitable
shipped in	ambient
storage temp.	room temp

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable