WTSIi202-A 66540437-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Depositor
Wellcome Trust Sanger Institute
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
PluriTest: Pluripotency score: 15.076
Novelty score: 1.41
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001001140
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Additional Cell Line Info,

EBiSC Access Use Agreement (EAUA),

Cell Line Information Pack (CLIP),

Note: EAUA and CLIP must be completed before order fulfillment
Medium: -
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 20
Temperature: 37°C
EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human dermis (fibroblast)
reprogramming method	Sendai virus
description	age (0-4)
Торговая марка	EBiSC™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture | stem cell: suitable
relevant disease(s)	diabetes (monogenic)
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable