UKKi021-A 66540547-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Depositor
Klinikum der Universitat zu Koln
Derivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SSEA-1 Yes -
TRA 1-80 Yes - -
CD90 Yes -
SOX2 Yes -
DNMT3b Yes -
NANOG Yes - -
OCT 4 Yes - -
FOXD3 Yes -
Morphology:
mophology pictures of NP0105-2 before banking and of the thawing control
mophology pictures of NP0105-2 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0105-2
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0105-2
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: Chr01: Trisomy of whole chromosome; Chr11: Deletion of telomeric region of long arm; Chr22: Onset of mosaicism at the telomeric end of long arm
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Additional Cell Line Info,
EBiSC Access Use Agreement (EAUA),
Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillment
Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 30
No. Vials banked (pre-EBiSC): 30
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (30-34)
Торговая марка	EBiSC™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture | stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable