CENSOi002-B 66540597-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Depositor
Censo Biotechnologies
Derivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes
SSEA-1 No
POU5F1 (OCT-4) Yes
SSEA-4 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
CXCR4 Yes
GATA6 Yes
SOX17 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
MIXL1 Yes
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 9
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: KaryoLite BoBs
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Additional Cell Line Info,

EBiSC Access Use Agreement (EAUA),

Cell Line Information Pack (CLIP),

Note: EAUA and CLIP must be completed before order fulfillment
Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.

biological source	human dermis (fibroblast)
reprogramming method	mRNA
description	age (5-9)
Торговая марка	EBiSC™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture | stem cell: suitable
relevant disease(s)	muscular dystrophy (Duchenne)
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable