SMC™ Human Total Tau High Sensitivity Immunoassay Kit 3-0185-00

The human Tau family of microtubule-associated neuronal proteins has been rigorously studied in the context of normal and aberrant neurobiology. While typically involved in the stability of axonal microtubules, Tau proteins can also play a role in neurodegenerative diseases such as Alzheimers Disease (AD) and frontotemporal dementia (FTD) if they aberrantly misfold and accumulate in the brain. There is significant interest in studying the level of Tau proteins in both the central nervous system as well as in circulation, and the new SMC™ Human Total-Tau High Sensitivity Immunoassay Kit enables researchers to take advantage of the Single Molecule Counting ultrasensitive immunoassay technology to perform accurately and sensitive measurements in human cerebrospinal fluid (CSF), plasma, and serum samples, toward making important discoveries about the role of Tau protein in neurodegenerative disease mechanisms.
The SMC™ Human Total Tau High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and TM="SMCxPRO"] immunoassay systems detect analytes using Single Molecule Counting SMC™ technology.
Specificity to the following species samples were tested with the follow results:

• Non Human Primate – 50%
• Mouse – 100%
• Rat – 100%
• Canine – 100%
• Feline – 75%

The sensitivity of the Single Molecule Counting SCM technology gives the SCM Human Total Tau High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Human Total Tau in human serum, plasma, and CSF.
This new streamline kit has the following features:

• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Able to achieve assay specifications on both SMCxPRO™ and Erenna.

The sensitivity of the Single Molecule Counting SMC™ technology gives the SMC™ Human Total Tau High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Human Total Tau in human serum, plasma, and CSF.
Research Category:
Neuroscience
neurological
Research Sub-Category:
Alzheimers Disease
Aging, Nervous System
Neurodegenerative Diseases
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neuroregenerative Medicine
Neuroscience

• Assay Buffer
• Total Tau Coated Beads
• Standard Diluent
• Total Tau Detection Antibody
• Total Tau Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Minimum of 3 serum samples spiked with average recovery of 70% with a range of 64-80%
Minimum of 4 plasma samples spiked with average recovery of 82% with a range of 76-88%
Minimum of 5 CSF samples spiked with average recovery of 91% with a range of 85-106%
SMC is a trademark of Singulex
SMCxPRO is a trademark of Merck KGaA, Darmstadt, Germany
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

species reactivity	human
Торговая марка	SMC™
assay range	sensitivity: 2.34 pg/mL ((lower limit of quantification)), inter-assay cv: 4% intra-assay cv: 8% (Intra-assay CV n=5; Inter-assay n=3)