Small Pack Antibodies
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Monoclonal Anti-Myocardin antibody produced in mouse
NACRES: NA.41
Кат. номер M8948-25UL M8948-200UL General descriptionMyocardin (MYOCD) is expressed in the muscles and cardiomyocytes. It is a co-transcriptional factor.
Monoclonal Anti-Myocardin (mouse IgG2b isotype) is derived from the hybridoma MCR54 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human myocardin. Myocardin (MYOCD) is a transcriptional coactivator.ApplicationMonoclonal Anti-Myocardin antibody produced in mouse has been used in western blot analysisimmunofluorescence.
Biochem/physiol Actions
Myocardin (MYOCD) takes part in the differentiation of vascular smooth muscle cells (VSMC). It is a co-factor of serum response factor (SRF) and regulates the activation of those genes which are dependent on SRF. MYOCD forms a complex with SRF on DNA.
Myocardin is a smooth and cardiac muscle-specific transcriptional coactivator of serum response factor (SRF). It regulates the expression of a set of cardiac and smooth muscle-specific genes. Myocardin was implicated in the pathogenesis of atherosclerosis, Alzheimer′s disease, and malignant transformation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone MCR54, monoclonal form buffered aqueous solution mol wt antigen ~150 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~2.0 mg/mL application(s) western blot: 2.5-5 µg/mL using HEK-293T cells overexpressing myocardin isotype IgG2b conjugate unconjugated UniProt accession no. Q8IZQ8, shipped in dry ice storage temp. 20°C Gene Information human ... MYOCD(93649)
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse
MDL number: MFCD00162703 NACRES: NA.41
Кат. номер M8421-25UL M8421-.2ML M8421-100UL M8421-.5ML General descriptionMyosin is a 480 kDa protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200 kDa each) and four light chains (15-26 kDa). Myosin 7 (MYH7) is mapped to human chromosome 14q11.1.
Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin, purified from myofibrils isolated from human skeletal muscle, was used as the immunogen.1-3. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2.
Specificity
Reacts with human adult skeletal muscle slow myosin. Does not react with the fast myosin heavy chain nor with embryonic or neonatal rat skeletal myosins, nor with cat fetal/embryonic skeletal myosin. Also reacts with human adult type I skeletal muscle fibers and with cardiac muscle cells. No reactivity detected with human or rat adult smooth muscle or with non-muscle tissues.
Monoclonal Anti-Myosin (Skeletal, Slow) recognizes an epitope located on the heavy meromyosin portion of human adult skeletal muscle slow myosin. Cross-reactivity has been observed with human, bovine, sheep, goat, porcine, dog, cat, rabbit, hamster, guinea pig, rat, mouse, and chicken. In rat, the product is highly specific for the slow myosin heavy chain of adult skeletal muscle. It does not react with the fast myosin heavy chain. It does not cross-react with embryonic or neonatal rat skeletal myosins, nor with cat fetal/embryonic skeletal myosin. Using immunohistochemical staining, the antibody reacts with human adult type I skeletal muscle fibers and with cardiac muscle cells. It also reacts faintly with adult human C-Fibers, co-expressing myosin heavy chain I and IIa in variable ratios. The product shows no reactivity with human or rat adult smooth muscle or with non-muscle tissues.ImmunogenHuman skeletal muscle myosin purified from myofibrils.ApplicationMonoclonal Anti-Myosin (Skeletal, Slow) may be used for the localization of slow (Type I) myosin.
Monoclonal Anti-Myosin (Skeletal, Slow) antibody has been used in the detection of Myosin 7 using:- light microscopy
- immunofluorescence staining
- immunoblotting
- ELISA
- solid-phase RIA
- immunohistology (frozen, formalin-fixed, paraffin-embedded and methacarn-fixed paraffin-embedded tissue sections)
- immunoelectronmicroscopy
Biochem/physiol Actions
Myosin molecules consist of two major regions: tail (rod) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type: skeletal, cardiac, smooth and in non-muscle. Myosin isoforms exist in different types of skeletal muscle, depending on the physiological function of the muscle. Mammalian muscle fibers are classified primarily into slow (I), fast-red (IIa) and fast-white (IIb) major types. Changes in the speed of muscle contraction brought about by neural influences result from changes in the pattern of expression of myosin and other myofibrillar genes.
Transient expression of different myosin isoforms occurs during fetal growth and development. Mutations in myosin 7 is associated with laing distal myopathy (LDM). Myosin 7 gene mutations results in muscular dystrophy diseases like scapuloperoneal myopathy. Mutations leads to storage of myosin protein aggregates in muscle, leading to myosin storage myopathy. Mutations in MYH7 is also associated with hypertrophic cardiomyopathy and in heart malformation disease called ebstein anomaly.
Physical form
Supplied as ascites fluid with 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form ascites fluid antibody product type primary antibodies clone NOQ7.5.4D, monoclonal contains 15 mM sodium azide species reactivity chicken, feline, bovine, guinea pig, canine, human, pig, goat, rabbit, sheep, mouse, rat, hamster packaging antibody small pack of 25 µL application(s) electron microscopy: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using protease-digested, sections of rabbit tongue","indirect ELISA: suitable","radioimmunoassay: suitable","western blot: 1:5,000 using extract of rat or rabbit tongue isotype IgG1 conjugate unconjugated UniProt accession no. P12883, shipped in dry ice storage temp. 20°C Gene Information human ... MYH7(4625)
mouse ... Myh7(140781)
rat ... Myh7(29557)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse
MDL number: MFCD00164708 NACRES: NA.41
Кат. номер N0142-25UL N0142-1VL N0142-100UL N0142-.2ML N0142-.5ML General descriptionNeurofilaments are composed of three subunits, namely NEFL (light), NEFM (medium) and NEFH (heavy). NEFH (neurofilament heavy) gene encodes the most important neuron-specific intermediate filament of cytoskeleton in myelinated axons. This gene is mapped to human chromosome 22q12.2.
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques.ImmunogenC-terminal segment of enzymatically dephosphorylated pig neurofilament 200.ApplicationMonoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody has been used in immunohistochemistry and western blotting.
Biochem/physiol Actions
The heavy polypeptide neurofilament encoded by neurofilament heavy chain (NEFH) gene participates in the maintenance of cytoskeleton and axonal architecture in the proximal axonal region of spinal motoneurons. Mutations in the NEFH gene has a key role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). NEFH gene is also identified to generate autosomal dominant axonal Charcot-Marie-tooth disease (CMT2cc).
Physical form
Supplied as ascites fluid containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200, biological source mouse antibody form ascites fluid antibody product type primary antibodies clone N52, monoclonal contains 15 mM sodium azide species reactivity wide range packaging antibody small pack of 25 µL application(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): 1:400 using rat cerebellum, microarray: suitable, western blot: 1:1,000 using rat brain extract isotype IgG1 conjugate unconjugated shipped in dry ice storage temp. 20°C Gene Information human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-Nitric Oxide Synthase, Inducible antibody produced in mouse
MDL number: MFCD00675308 NACRES: NA.41
Кат. номер N9657-25UL N9657-.2ML General descriptionMonoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) (mouse IgG1 isotype) is derived from the NOS-IN hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. Nitric oxide synthase (NOS) is classified into three types based on molecular mass, subcellular location, and Ca2+ dependence. Type I NOS (150-160 kDa) is found in neurons. It is also called as NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kDa), best characterized in macrophages, also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kDa) is found in endothelial cells. It is called as endothelial NOS (eNOS, or ecNOS). Type II NOS is Ca2+ -independent and is expressed in activated macrophages and some glial cells after stimulation. iNOS is also found in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts.
Specificity
Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS), antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.Immunogensynthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) from mouse macrophage (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.ApplicationMonoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) has been used in- immunofluorescence staining
- immunogold electron microscopy
- immunoblotting
- immunohistochemistry
- immunocytochemistry
- enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Nitric oxide synthase (NOS) is an enzyme involved in the synthesis of nitric oxide (NO), a free radical generated under physiological conditions by virtually all mammalian cells. NO is formed from arginine by NOS which oxidizes a guanidino nitrogen of arginine, releasing NO and citrulline. NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. Various types of NOS may serve a variety of diverse biological pathways.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form ascites fluid antibody product type primary antibodies clone NOS-IN, monoclonal mol wt antigen 130 kDa contains 15 mM sodium azide species reactivity rat, mouse packaging antibody small pack of 25 µL application(s) microarray: suitable","western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN- isotype IgG1 conjugate unconjugated UniProt accession no. Q06518, shipped in dry ice storage temp. 20°C Gene Information human ... NOS2(4843)
mouse ... Nos2(18126)
rat ... Nos2(24599)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-Notch 1 antibody produced in rat
NACRES: NA.41
Кат. номер SAB4200024-25UL SAB4200024-200UL General descriptionMonoclonal Anti-Notch 1 (rat IgG2b isotype) is derived from the hybridoma ICN 5B5 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant fusion protein corresponding to a fragment of human Notch 1 fusion protein. Notch receptor 1 (Notch1) gene is located on human chromosome 9q34.ApplicationMonoclonal Anti-Notch 1 antibody produced in rat has been used in immunoblotting.
Biochem/physiol Actions
In chronic obstructive pulmonary disease, notch receptor 1 (Notch1) helps to modulate endothelial apoptosis via the extracellular signal-regulated kinase (ERK) pathway. Overexpression of Notch1 helps to guard endothelial cell from apoptosis. Knockdown of Notch1 results in the enhancement of apoptotic cells. Notch1 serves as a tumor suppressor gene in mammalian epidermis and is a determinant of keratinocyte differentiation. Notch signaling is believed to participate in several human neoplasms including T-cell acute lymphoblastic leukemia.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone ICN 5B5, monoclonal form buffered aqueous solution mol wt antigen ~120 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) western blot: 2-4 µg/mL using whole extract of human Jurkat cells and developing with a sensitive film isotype IgG2b conjugate unconjugated UniProt accession no. P46531, shipped in dry ice storage temp. 20°C Gene Information human ... NOTCH1(4851)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-Notch3 antibody produced in rat
NACRES: NA.41
Кат. номер SAB4200125-25UL SAB4200125-200UL General descriptionMonoclonal Anti-Notch3 (rat IgG2a isotype) is derived from the hybridoma NIZ 8G5 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from the immunized rat.
Members of the Notch gene family encode transmembrane receptors. Mammals have four Notch receptors, Notch1 through Notch4. Notch1 and Notch2 have the highest homology with each other, whereas Notch3 and Notch4 are structurally divergent, lacking the transactivation domain that is found in Notch1 and Notch2. The Notch3 receptor possesses 34 extracellular epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN12 repeats (LIN). It also contains two protein-protein interaction domains, the RAM domain (R), two nuclear localization signals (NLS), six ankyrin (ANK) repeats and a PEST sequence (P) at its cytoplasmic portion. The NOTCH3 gene is mapped to human chromosome 19p13.12.
Specificity
Monoclonal Anti-Notch3 recognizes human and rat Notch3.Immunogenhuman recombinant full length Notch3.ApplicationMonoclonal Anti-Notch3 antibody produced in rat is suitable for immunoblotting.
Biochem/physiol Actions
The Notch receptors are critical for various cell fate decisions. The Notch signaling plays a key role in the normal development of many tissues and cell types. It has diverse effects on differentiation, survival and/or proliferation that are highly dependent on the signal strength and cellular context. Variations in the NOTCH3 gene leads to vascular degenerative disease known as cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). In addition, upregulation of the NOTCH3 gene indicates active and relapsing human T-cell acute lymphoblastic leukemia (T-ALL).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone monoclonal form buffered aqueous solution mol wt antigen ~270 kDa species reactivity rat, human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) western blot: 2-4 µg/mL using using Jurkat cell extracts isotype IgG2a conjugate unconjugated UniProt accession no. Q9UM47, shipped in dry ice storage temp. 20°C Gene Information human ... NOTCH3(4854)
rat ... Notch3(56761)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-NUP153 antibody produced in rat
NACRES: NA.41
Кат. номер N6663-25UL N6663-200UL General descriptionNucleoporin 153kDa (NUP153) has a NE (nuclear envelope)-and NPC (nuclear pore complex)-targeting site, four zinc-fingers and a phenylalanine-glycine-repeat. It is part of the FG repeat class of proteins. The gene encoding it is localized to human chromosome 6p22.3.Immunogenrecombinant protein corresponding to amino acids 610-1191 of rat Nup15.ApplicationMonoclonal Anti-NUP153 antibody produced in rat has been used to study binding protein partners for thyroid hormone receptor-β2.
Biochem/physiol Actions
Nucleoporin 153kDa (NUP153) is a nuclear pore protein crucial for nuclear import. It is involved in importin α/β-mediated import and mRNA export from the nucleus. NUP153 is involved in HIV (human immunodeficiency virus)-1 nuclear import and integration. Poliovirus and rhinovirus infection results in the cleavage of this protein, thereby disrupting nucleo-cytoplasmic trafficking. Similarly, cardiovirus infection results in NUP153 phosphorylation, thereby modulating nuclear pore complex. NUP153 also has a role in cell cycle modulation and apoptosis. It associates with lamin B and might have a role in the anchoring of nuclear pore complexes to the nuclear lamina.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone R3G1, monoclonal form buffered aqueous solution mol wt antigen apparent mol wt ~160 kDa","antigen predicted mol wt ~153 kDa species reactivity primate, rat, mouse, human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) immunocytochemistry: suitable","immunohistochemistry: suitable","western blot: 2-4 µg/mL using HeLa total cell extract isotype IgG2a conjugate unconjugated UniProt accession no. P49790, shipped in dry ice storage temp. 20°C Gene Information human ... NUP153(9972)
mouse ... Nup153(218210)
rat ... Nup153(25281)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-NY-ESO-1 antibody produced in mouse
MDL number: MFCD09265426 NACRES: NA.41
Кат. номер N2038-100UL N2038-25UL N2038-200UL General descriptionMonoclonal Anti-NY-ESO-1 (mouse IgG1 isotype) is derived from the hybridoma E978 produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with human recombinant NY-ESO-1. The NY-ESO-1, also known as cancer/testis antigen 1 is expressed in normal testis and to a much lower extent in placenta, ovary and uterus.Immunogenrecombinant human NY-ESO-1.ApplicationMonoclonal Anti-NY-ESO-1 antibody produced in mouse has been used in enzyme linked immunosorbent assays (ELISA), immunoblotting and immunohistochemistry.
Monoclonal Anti-NY-ESO-1 is used as a probe to determine the presence and roles of Cancer/Testis Antigen 1 in humoral immune responses and a variety of cancers.
Biochem/physiol Actions
Cancer/testis (CT) antigens are the protein products of genes that are activated in a wide variety of tumors and can elicit autologous cellular and humoral immune responses. Their normal expression is restricted to male germ cells in the testis and they are not expressed in adult somatic tissues. Therefore, CT antigens are promising candidates for cancer immunotherapy. Approximately 20 CT antigens or antigen families have been identified to date. NY-ESO-1 is a CT protein to which cell-mediated immune response has been demonstrated. It is highly expressed in various types of cancers. For example, patients with melanoma contain tumor-infiltrating lymphocytes cells (TILs) that recognized NY-ESO-1 protein. The expression of genes encoding CT antigens was examined in sporadic medullary thyroid carcinoma. The expression of NY-ESO-1 was found in 65 percent of the samples and correlated with tumor recurrence in primary MTC tumors. Furthermore, high frequency of expression was found in breast cancer especially in benign lesions and in multiple myeloma. Due to its wide expression in different cancers, NY-ESO-1 protein was used as a vaccine in cancer patients.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source mouse Quality Level 300, antibody form purified immunoglobulin antibody product type primary antibodies clone E978, monoclonal form buffered aqueous solution mol wt antigen ~23 kDa species reactivity human packaging antibody small pack of 25 μL concentration ~1 mg/mL application(s) immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 1-2 μg/mL using SKMel-37 total cell extract isotype IgG1 conjugate unconjugated shipped in dry ice storage temp. −20°C Gene Information human ... CTAG1B(1485)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-O-Glycosylated Neurofilament-M antibody produced in mouse
MDL number: MFCD09265427 NACRES: NA.41
Кат. номер G9670-25UL G9670-200UL General descriptionIntermediate filaments (IFs) have a diameter of 8-10nm and differ from microtubules and microfilaments significantly. Neurofilaments (NFs) are a type of IFs found mainly in cells or tissues of neuronal origin. NFs are composed of three major proteins, NF-L, NF-M and NF-H, having a molar mass of 68kDa, 160kDa, and 200kDa, respectively.
Monoclonal Anti-O-Glycosylated Neurofilament-M (mouse IgG2a isotype) is derived from the hybridoma NL6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a protein fraction enriched for components of the human neuronal cytoskeleton. Neurofilament medium (NEFM) gene encodes neurofilament medium polypeptide which constitutes to cytoskeleton of mature neurons. The gene in human chromosome is localized on 8p21.Immunogenprotein fraction enriched for components of the human neuronal cytoskeleton from cell lines NT2-N and SK-N-BE(2). The epitope is localized in the carboxy-terminal tail domain of NF-M.ApplicationMonoclonal Anti-O-Glycosylated Neurofilament-M antibody produced in mouse has been used in:- immunohistochemical methods
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
The NF proteins are synthesized in the neuronal perikarya before assemby to form the filaments. These filaments are then transported within the axons towards the synaptic terminals. The function of these NF polypeptides is regulated by phosphorylation, which influences their interaction with cytoplasmic organelles. The head region of NF-L and NF-M contain sites modified by O-linked GlcNAc. Down-regulation of NF-M O-GlcNAcylation and increased phosphorylation of NF-M is observed in Alzheimer disease. This antibody recognizes specifically an O-glycosylated epitope in the projection domain of NF-M. In a rat model for amyotrophic lateral sclerosis (ALS), this specific modification is strongly decreased compared with wild-type animals.
Neurofilament medium (NEFM) is essential for radial growth of axon which includes the diameter of the axon as well as the myelination. The C-terminus of NFEM gene has 7 repeats of KSP (lysine-serine-proline) domain and phosphorylation of which is crucial for regulating the radial growth of axon.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone NL6, monoclonal form buffered aqueous solution mol wt antigen ~160 kDa species reactivity rat, human should not react with mouse, bovine packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunocytochemistry: suitable","immunohistochemistry: suitable","western blot: 0.5-1 µg/mL using human NT2 cell extracts conjugate unconjugated UniProt accession no. P07197, shipped in dry ice storage temp. 20°C Gene Information human ... NEFM(4741)
rat ... Nefm(24588)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-OSTF1 antibody produced in mouse
NACRES: NA.41
Кат. номер SAB4200122-25UL SAB4200122-200UL General descriptionMonoclonal Anti-OSTF1 (mouse IgG1 isotype) is derived from the hybridoma OSTF103 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Osteoclast-stimulating factor (OSF or OSTF1) is an intracellular protein, highly expressed in osteoclasts. This protein is composed of a Src homology 3 (SH3) domain followed by three ankyrin repeats. OSTF1 also contains proline and aspartate largely at the N-terminal and C-terminal regions respectively. The gene OSTF1 is mapped to human chromosome 9q21.13.
Specificity
Monoclonal Anti-OSTF1 recognizes human, monkey, dog, bovine, rat, and mouse OSTF1.ApplicationMonoclonal Anti-OSTF1 antibody produced in mouse is suitable for immunoblotting.
Biochem/physiol Actions
Osteoclast-stimulating factor (OSF or OSTF1) indirectly enhances osteoclast (OCL) formation and bone resorption through a cellular signal transduction cascade. This may be possible through its interaction with c-Src or other c-Src-related proteins. The domain structure of OSTF1 suggests that it potentially participates in protein-protein interactions. The Src homology 3 (SH3) domain is known to associate with survival motor neuron (SMN), a spinal muscular atrophy disease determining gene product. This interaction results in the stimulation of OCL formation. Inferring that OSF SH3-SMN interaction may be critical in congenital bone fractures associated with type I spinal muscular atrophy disease. Hindrance in the OSF-SH3 domain interaction with its protein partners might decrease bone resorption by OCL. This could prevent the bone loss associated with many bone diseases, such as osteoporosis, periodontal disease, estrogen deficiency, inflammatory bone loss, hyperparathyroidism, bone malignancy and Pagets disease.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source mouse antibody form affinity isolated antibody antibody product type primary antibodies clone OSTF1, monoclonal form buffered aqueous solution mol wt antigen ~28 kDa species reactivity human, canine, mouse, rat, monkey, bovine packaging antibody small pack of 25 µL concentration ~1.5 mg/mL application(s) western blot: 2-4 µg/mL using A431, Hepa1-6, NRK or PC12 cell extracts conjugate unconjugated UniProt accession no. Q92882, shipped in dry ice storage temp. 20°C Gene Information human ... OSTF1(26578)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-PAX9 antibody produced in rat
NACRES: NA.41
Кат. номер SAB4200083-25UL SAB4200083-200UL General descriptionMonoclonal Anti-PAX9 (rat IgG1 isotype) is derived from the hybridoma paired box 9 (PAX9) 7C2 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant fragment corresponding to a fragment of mouse PAX9 coupled to myelin basic protein (MBP). The PAX gene family encodes a group of transcription factors containing a 128-aa paired DNA-binding domain, and in some cases, a paired-type homeodomain. Nine PAX genes (PAX1-PAX9) has been observed in mammals. Pax9 and Pax1 show high similarities. Pax9 is found in all vertebrates including chick, zebrafish, mouse and man.ApplicationMonoclonal Anti-PAX9 antibody produced in rat has been used immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Paired box 9 (PAX9) protein is known to play a role in the formation of various organs and skeletal elements. Mutations in either the PAX1 or the PAX9 genes may produce an inherited skeletal disorder such as the Jarcho-Levin syndrome. Interestingly, progressive loss of PAX9 expression was correlated with increasing malignancy of dysplastic and cancerous epithelium of human esophagus and lung cancer.
Target description
Anti-PAX9, paired box 9, is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal de
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone PAX9 7C2, monoclonal form buffered aqueous solution mol wt antigen ~36 kDa species reactivity mouse, human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) immunohistochemistry: suitable","western blot: 0.25-0.5 µg/mL using H1299 cell extracts isotype IgG1 conjugate unconjugated UniProt accession no. P55771, shipped in dry ice storage temp. 20°C Gene Information mouse ... Pax9(18511)
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Monoclonal Anti-Phosphacan antibody produced in mouse
MDL number: MFCD09265428 NACRES: NA.41
Кат. номер P8874-25UL P8874-200UL General descriptionMonoclonal Anti-Phosphacan (mouse IgM isotype) is derived from the hybridoma 122.2 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with rat brain proteoglycans. Chondroitin sulfate proteoglycans are neural cell adhesion molecules (NCAM) ligands present in the brain extracellular matrix (ECM). Phosphacan protein is expressed mainly in astrocytes and is a ligand for NCAM. Phosphacan is the soluble extracellular domain of the receptor-type transmembrane protein tyrosine phosphatase (RPTPb).Immunogenrat brain proteoglycans.ApplicationMonoclonal Anti-Phosphacan antibody produced in mouse has been used in:- immunoblotting
- immunohistochemistry
- immunocytochemistry.
Biochem/physiol Actions
Phosphacan levels elevates during late embryogenesis. It reaches a plateau two weeks postnatal before reaching stable. Receptor-type transmembrane protein tyrosine phosphatase (RPTPb) functions to promote primary tecal neurons neurite growth, neural migration and also induces cell adhesion. Both phosphacan and RPTPb can bind to NCAM and tenascin-C and −R. Phosphacan can oppose RPTPb by competing for its binding sites. Both in hippocampal and spinal cord neurons, phosphacan can affect neuronal adhesion and neurite outgrowth.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 122.2, monoclonal form buffered aqueous solution mol wt antigen ~180 kDa (higher band may be present) species reactivity rat packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunocytochemistry: suitable","immunohistochemistry: suitable","western blot: 0.2-0.4 µg/mL using total extract of rat brain isotype IgM conjugate unconjugated UniProt accession no. Q62656, shipped in dry ice storage temp. 20°C Gene Information rat ... Ptprz1(25613)
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Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat
MDL number: MFCD03457568 NACRES: NA.41
Кат. номер H9908-.2ML H9908-25UL General descriptionHistone H1 and the N-terminal tail of histone-3 (H3) have key roles in the folding and inter-association of the chromatin fibers. Two currently known phosphorylation sites are present in the N-terminus of H3; serine-10 and serine-28. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). Histone H3 is located on human chromosome 6p22.
Specificity
The antibody does not detect the unphosphorylated epitope. It detects the histone molecule at the onset of mitosis (prophase, metaphase, weaker at the beginning of anaphase, but not during late anaphase).
Monoclonal Anti-Phospho-Histone H3 (pSer28) reacts specifically with histone H3 phosphorylated at Ser28 near its N-terminus, and does not detect the unphosphorylated epitope. It detects the phosphorylated histone molecule at the onset of mitosis (prophase, metaphase and weaker at the beginning of anaphase), but not during late anaphase. Reactivity has been observed with human, bovine, hamster and mouse.ImmunogenSynthetic peptide corresponding to amino acids of human histone H3.ApplicationMonoclonal Anti-phospho-Histone H3 (pSer28) antibody is suitable for immunoblotting (approx. 15 kDa), immunocytochemistry (3.7% formaldehyde-methanol fixation) and flow cytometry applications.
Biochem/physiol Actions
Mitogenic stimulation, oncogenic transformation, or induction of oncogenic ras expression are accompanied with increase in serine-10 phosphorylation of the H3 N-terminal domain. It has been shown that phosphorylated H3 is associated with c-fos and c-myc genes in stimulated cells. Phosphorylation of H3, at both serine-10 and -28, coincides with the induction of mitotic chromosome condensation.
H3 phosphorylation may contribute to proto-oncogene induction by modulating chromatin structure and releasing blocks in elongation. H3 dephosphorylation occurs quite rapidly after mitosis and serine-10/28 reo main unphosphorylated throughout the remainder of interphase. PP1 has been identified as the H3 phosphatase.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor
Storage and Stability
For continuous use, store at 2 to 8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone HTA28, monoclonal form buffered aqueous solution mol wt antigen 15 kDa species reactivity hamster, mouse, human, bovine packaging antibody small pack of 25 µL concentration ~0.5 mg/mL application(s) flow cytometry: suitable","immunocytochemistry: suitable using 3.7% formaldehyde-methanol fixation","microarray: suitable","western blot: 0.5-1 µg/mL using whole extract of cultured human acute T cell leukemia Jurkat cells treated with Nocodazole. isotype IgG2a conjugate unconjugated UniProt accession no. P68431, shipped in dry ice storage temp. 20°C Gene Information human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
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Monoclonal Anti-phospho-MDMX (pTyr55) antibody produced in mouse
NACRES: NA.41
Кат. номер M8070-25UL M8070-200UL General descriptionMonoclonal Anti-phospho-MDMX (pTyr55) (mouse IgG2a isotype) is derived from the hybridoma PH-MDMX-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse Double Minute 4 (MDM4) comprises the N-terminal p53 binding domain, a zinc finger, and an acidic region, followed by a really interesting new gene (RING) domain at C-terminus. MDM4 gene is mapped to human chromosome 1q32.1. The RING finger harbors a putative nuclear localization signal.
Specificity
Monoclonal Anti-phospho-MDMX (pTyr55) recognizes human phosphorylated MDMX (pTyr55) (approx. 80 kDa).Immunogensynthetic phosphopeptide corresponding to amino acids 50-64 (pTyr55) of human MDMX.ApplicationMonoclonal Anti-phospho-MDMX (pTyr55) antibody produced in mouse may be used in:- immunoblotting
- immunoprecipitation
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Mouse Double Minute 4 (MDM4 or HDMX or MDMX) undergoes phosphorylation. It is the major inhibitor of p53 transcriptional activity. Despite their significant structural similarity, mouse double minute 2 (MDM2) and MDMX play distinct roles in p53 regulation. The critical and non-redundant role of MDMX and MDM2 in the regulation of p53 in vivo is strikingly demonstrated by the lethality of the mdmx or mdm2 null embryos, which can be fully rescued by the elimination of p53. MDM2 or MDMX are frequently overexpressed in human cancers, leading to the suppression of p53. Studies on MDMX in response to stress conditions, involving post-translational modifications and protein-protein interactions are reported.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone PH-MDMX-55, monoclonal form buffered aqueous solution mol wt antigen ~80 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) indirect ELISA: suitable","western blot: 4-8 µg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase isotype IgG2a conjugate unconjugated UniProt accession no. O15151, shipped in dry ice storage temp. 20°C Gene Information human ... MDM4(4194)
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Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse
NACRES: NA.41
Кат. номер M8195-200UL M8195-25UL General descriptionMonoclonal Anti-phospho-MDMX (pTyr99) (mouse IgG2b isotype) is derived from the hybridoma PH-MDMX-169 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse double minute 4 (MDM4) or MDMX comprises p53 binding domain, central acidic region, zinc and interesting new gene (RING) finger domain and is a phosphorylated protein. MDM4 gene is mapped to human chromosome 1q32.1.
MDMX is a nuclear protein and the gene encoding it is localized on human chromosome 1q32.1.
Specificity
Monoclonal Anti-phospho-MDMX (pTyr99) recognizes human phosphorylated MDMX (pTyr99) (~ 80 kDa). The antibody does not recognize the non-phosphorylated MDMX, which is produced when MDMX is expressed with a kinase mutant.Immunogensynthetic phosphopeptide corresponding to amino acids 93-106 (pTyr99) of human MDMX.ApplicationMonoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse may be used in:- enzyme-linked immunosorbent assay (ELISA)
- immunofluorescence
- immunoblotting
Biochem/physiol Actions
Studies began to unravel the regulation of murine double minute X (MDMX) in response to stress conditions, involving post-translational modifications and protein-protein interactions.
MDMX associates with tumor suppressor p53 and inhibits its activity. It is upregulated in many tumors.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone PH-MDMX-169, monoclonal form buffered aqueous solution mol wt antigen ~80 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) indirect ELISA: suitable","indirect immunofluorescence: suitable","western blot: 1-2 µg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase isotype IgG2b conjugate unconjugated UniProt accession no. O15151, shipped in dry ice storage temp. 20°C Gene Information human ... MDM4(4194)
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Monoclonal Anti-Phosphoserine antibody produced in mouse
Кат. номер P5747-25UL P5747-100UL P5747-200UL
Specificity
By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.Immunogenphosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).ApplicationThe antibody has been used for the detection of some phosphoserine containing proteins using immunoblotting and dot blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
Biochem/physiol Actions
Protein phosphorylation and dephosphorylation are basic signaling mechanisms that modify protein function in eukaryotic cells. Phosphorylation is a rare posttranslational event in normal tissues, however, the abundance of phosphorylated cellular proteins increases several folds following various activation processes. The main amino acids that are phosphorylated are tyrosine, serine, or threonine (pTyr/pSer/pThr), each having specific kinases that phosphorylate them and specific phosphatases that dephosphorylate them. Mono and polyclonal antibodies directed against phosphorylated residues were generated and found useful as analytical and preparative tools by enabling the identification, quantification, and immunoaffinity isolation of phosphorylated cellular proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone PSR-45, monoclonal form buffered aqueous solution packaging antibody small pack of 25 μL concentration ~2 mg/mL application(s) dot blot: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, microarray: suitable, western blot: 2.5-5.0 μg/mL using total rat brain extract. isotype IgG1 conjugate unconjugated shipped in dry ice storage temp. −20°C
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Monoclonal Anti-PIASy antibody produced in mouse
Кат. номер P0104-25UL P0104-200UL General descriptionMonoclonal Anti-PIASy (mouse IgG1 isotype) is derived from the hybridoma PIA4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Protein inhibitor of activated STAT4 (PIASy/PIAS4) belongs to the protein inhibitor of activated STAT (PIAS) family. It codes for a RING finger (RNF) protein. This gene is located on human chromosome 9p13.3.
Specificity
Monoclonal Anti-PIASγ recognizes human PIASγ.Immunogensynthetic peptide corresponding to amino acids 491-504 of human PIASy.ApplicationMonoclonal Anti-PIASy antibody produced in mouse may be used in:- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
The protein inhibitor of activated STAT (PIAS) family of proteins are known to acts as small ubiquitin-related modifier (SUMO)−E3 ligase. PIASy can catalyze sumoylation of lymphoid enhancer factor 1 (LEF1). In addition, PIASy may have a direct role in the execution of the senescence program in cells. Thus PIASy has different regulatory mechanisms on different protein substrates. It serve as a negative modulator of innate immunity. PIAS4 is known to participate in ubiquitin signaling pathways. It has been found that sumoylation of p53 by PIASy regulates the interaction of p53 with MDM2 and drives the export of p53 from the nucleus to the cytoplasm.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone PIA4, monoclonal form buffered aqueous solution mol wt antigen ~70 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~1.5 mg/mL application(s) immunocytochemistry: suitable","western blot: 1-2 µg/mL using total cell extract of HEK-293T cells transfected with human PIASy isotype IgG1 conjugate unconjugated UniProt accession no. Q8N2W9, shipped in dry ice storage temp. 20°C Gene Information human ... PIAS4(51588)
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Monoclonal Anti-Pinin antibody produced in mouse
NACRES: NA.41
Кат. номер P0084-200UL P0084-25UL General descriptionMonoclonal Anti-Pinin (mouse IgG1 isotype) is derived from the hybridoma 5F1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Pinin (PNN) is a serine/arginine-rich (SR)-related protein, that is found to localize to the cytoplasmic face of the desmosomal plaque in the convergence of intermediate filaments and to pin them to the desmosome. It consists of an Arg-Ser (RS) domain in its carboxyl terminus. This gene is mapped to human chromosome 14q.
Specificity
Monoclonal Anti-Pinin recognizes human pinin. It crossreacts with monkey, bovine, and dog pinin.Immunogensynthetic peptide corresponding to amino acids 547-737 of human pinin, conjugated to KLH.ApplicationMonoclonal Anti-Pinin antibody produced in mouse may be used in:- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Pinin (PNN) is mainly linked with the mature desmosomes of the epithelia. It plays a role in inducing junction formation and enhance cell aggregation. PNN transcription and protein levels were found to be reduced in renal and transitional cell carcinomas, suggesting its role as a tumor suppressor. PNN protein reduction by RNAi or knockdown resulted in loss of cell-cell adhesion as well as aberrant mouse development. It plays a key role in mRNA export, cell−cell adhesion, alternative splicing and cell migration. PNN also controls gene transcription.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if notused within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 5F1, monoclonal form buffered aqueous solution mol wt antigen ~140 kDa species reactivity canine, human, bovine, monkey packaging antibody small pack of 25 µL concentration ~1.5 mg/mL application(s) immunocytochemistry: suitable","indirect ELISA: suitable","western blot: 1-2 µg/mL using HeLa nuclear cell extract isotype IgG1 conjugate unconjugated UniProt accession no. Q9H307, shipped in dry ice storage temp. 20°C Gene Information human ... PNN(5411) Узнать цену -
Monoclonal Anti-PMP22 antibody produced in mouse
Кат. номер P0081-25UL P0081-200UL General descriptionPeripheral myelin protein 22 (PMP22), also known as growth arrest-specific protein 3 (GAS-3), is a tetraspan glycoprotein. It is highly expressed in myelin-forming Schwann cells of peripheral nerves. The gene encoding it is localized on human chromosome 17p12–13.Immunogenpeptide corresponding to the second extracellular domain of human PMP22 (amino acids 120-133).
Biochem/physiol Actions
Peripheral myelin protein 22 (PMP22) plays a vital role in myelination during peripheral nerve development. It also has a role in cell-cell interactions, cell proliferation, maintenance of axons and the determination of myelin thickness and stability. Aberrations or mutations in the PMP22 gene lead to heritable demyelinating peripheral neuropathies, such as Charcot-Marie-tooth disease type IA (CMT1A) and Dejerine-Sottas syndrome. Overexpression of PMP22 might contribute to the development of chronic myeloid leukemia (CML).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone CF1, monoclonal form buffered aqueous solution mol wt antigen ~22 kDa species reactivity monkey, human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) immunocytochemistry: suitable","immunohistochemistry: 2.5-5 µg/mL using human spinal cord tissue sections.","indirect ELISA: suitable","western blot: suitable isotype IgG1 conjugate unconjugated UniProt accession no. Q01453, shipped in dry ice storage temp. 20°C Gene Information human ... PMP22(5376)
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Monoclonal Anti-Polyglutamines antibody produced in mouse
MDL number: MFCD01322176 NACRES: NA.41
Кат. номер P1874-25UL P1874-200UL P1874-100UL General descriptionAbnormal stretch of triplet CAG (or CAA) codon produces a homomeric repeat of glutamine residues beyond the critical threshold and will lead to neurodegenerative disease. Monoclonal mouse anti-polyglutamine antibody can be used to detect poly Q protein expression and in western blot. Monoclonal anti-polyglutamines antibody reacts specifically to homomeric polyglutamines independent of the species.ImmunogenGST-human Huntingtin (N-terminal fragment of 171 amino acids containing 65Q).ApplicationApplications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
Monoclonal anti-polyglutamines antibody may be used for ELISA, immunoblot and slot blot. It can also be used in immunoprecipitation and immunocytochemistry.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.Legal InformationThis product is for in vitro use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California. This product is sold under license to U.S. Patent No. 6,291,652, owned by the Regents of the University of California.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 3B5H10, monoclonal form buffered aqueous solution species reactivity human packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: 1-2 µg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch isotype IgG1 conjugate unconjugated UniProt accession no. P42858, shipped in dry ice storage temp. 20°C Gene Information human ... HTT(3064)
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Monoclonal Anti-polyHistidine antibody produced in mouse
Кат. номер SAB4200620-200UL SAB4200620-25UL General descriptionMonoclonal Anti-polyHistidine (mouse IgG2a isotype) is derived from the HIS-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant His-tagged fusion protein. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. Monoclonal anti-polyHistidine recognizes native as well as denatured, reduced forms of synthetic polyHistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET, and pTrc expression vectors. The antibody preferentially recognizes N-terminal tagged fusion proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.Other NotesIn order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration. Use of sensitive film is recommended.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone HIS-1, monoclonal packaging antibody small pack of 25 μL concentration ~1.0 mg/mL application(s) flow cytometry: 3-6 μg/test, immunoblotting: 0.25-0.5 μg/mL, immunofluorescence: 4-8 μg/mL isotype IgG2a storage temp. −20°C
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Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse
NACRES: NA.41
Кат. номер P8825-.2ML P8825-100UL P8825-25UL P8825-.5ML General descriptionPCNA (proliferating cell nuclear antigen) is a member of the structurally and functionally conserved family of DNA sliding clamps (β clamps). They exist as ring-shaped complexes- homotrimers in eukaryotes, having pseudohexameric symmetry. A monomer of PCNA is composed of two similar globular domains, connected by an interdomain connecting loop, which is a long, and probably a flexible loop. These monomers organize themselves in a head-to-tail manner to form a ring, which has an inner positively charged surface composed of α helices and an outer surface of β sheets.
Monoclonal Anti-Proliferating Cell Nuclear Antigen (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with PCNA-Protein A fusion protein. Proliferating cell nuclear antigen (PCNA, 36 kDa), also known as cyclin, is an auxiliary protein of DNA polymerase. The protein is present in the nucleoplasm of continually cycling cells throughout the cell cycle.
Specificity
Recognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues.ImmunogenPCNA-Protein A fusion proteinApplicationMonoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used in:- enzyme linked immuno sorbent assay (ELISA)
- immunoblotting
- immunohistology
- immunoprecipitation
- flow cytometry
Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used for immunohistochemistry (IHC) Western blotting (WB).
Biochem/physiol Actions
Proliferating cell nuclear antigen (PCNA) is essential for DNA replication during S-phase. It is essential for cellular DNA synthesis. PCNA is required for leading strand synthesis in the SV40 system where it acts as an auxiliary protein for polymerase.
PCNA (proliferating cell nuclear antigen) is loaded around the template-primer 3′ ends, which is recognized by the conserved chaperone-like complex RFC (replication factor C), and this mechanism is ATP-dependent. Encircling of the DNA by the PCNA ring ensures firm anchoring of the polymerases to the DNA, and hence, functions as a co-factor for DNA polymerases. Post-translation modifications of this protein at the K164 residue is required for the regulation of DNA damage tolerance (DDT) pathways, pathways that ensure recovery from replication arrest at DNA lesions.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form ascites fluid antibody product type primary antibodies clone PC 10, monoclonal contains 15 mM sodium azide species reactivity rat, mouse","yeast, monkey, insect, human packaging antibody small pack of 25 µL application(s) flow cytometry: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using human tonsil sections","immunohistochemistry (frozen sections): suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 1:3,000 using HS-68 human foreskin- cell extract isotype IgG2a conjugate unconjugated UniProt accession no. P04961,","P12004, shipped in dry ice storage temp. 20°C Gene Information human ... PCNA(5111)
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Monoclonal Anti-PSF antibody produced in mouse
MDL number: MFCD03457573 NACRES: NA.41
Кат. номер P2860-25UL P2860-.2ML P2860-100UL General descriptionMonoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.Immunogenlysate of mouse bone marrow-derived stromal cell line.ApplicationApplications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper),
Immunohistochemistry (1 paper),
Immunoprecipitation (1 paper),
Monoclonal Anti-PSF antibody produced in mouse has been used in:- immunofluorescence
- immunohistochemistry
- immunoprecipitation
Biochem/physiol Actions
Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone B92, monoclonal form buffered aqueous solution species reactivity mouse, human packaging antibody small pack of 25 μL concentration ~2 mg/mL application(s) immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, radioimmunoassay: suitable using cells, western blot: 2-4 μg/mL using whole extract of cultured HeLa cells isotype IgG1 conjugate unconjugated storage temp. −20°C
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Monoclonal Anti-Rab7 antibody produced in mouse
MDL number: MFCD08705596 NACRES: NA.41
Кат. номер R8779-100UL R8779-200UL R8779-25UL General descriptionRab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). This family belongs to the rat sarcoma (Ras) superfamily of small GTPases. Rab proteins contain conserved regions involved in guanine nucleotide binding and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. The gene Rab7a in human chromosome is mapped to 3q21.3.
Specificity
Monoclonal Anti-Rab7 recognizes human, monkey, bovine, rat, mouse, and chicken Rab7.ApplicationMonoclonal Anti-Rab7 antibody produced in mouse may be used in ELISA, immunoblotting (~24 kDa), immunoprecipitation, immunohistochemistry, immunofluorescence and immunocytochemistry.
Biochem/physiol Actions
Rab GTPases are central regulators of membrane trafficking between different subcellular compartments in eukaryotic cells. The regulatory capacity of Rab GTPases depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Activation of Rab proteins is coupled to their association with intracellular membranes, allowing them to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin and tubulin-dependent vesicle movement, and membrane fusion. Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are Rab-interacting lysosome protein (RILP) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rab-7-interacting ring finger protein (Rabring7) and the hVPS34/p150 complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone Rab7-117, monoclonal form buffered aqueous solution mol wt ~24 kDa species reactivity human, chicken, rat, mouse, bovine, monkey packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunocytochemistry: suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.5-1 µg/mL using HeLa total cell extract isotype IgG2b conjugate unconjugated UniProt accession no. P51149, shipped in dry ice storage temp. 20°C Gene Information human ... RAB7A(7879)
mouse ... Rab7(19349)
rat ... Rab7a(29448)
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Monoclonal Anti-RNF4 antibody produced in mouse
Кат. номер SAB4200645-25UL SAB4200645-100UL General descriptionRNF4 (ring finger protein 4), initially identified as a transcriptional cofactor, is an E3 ubiquitin ligase. As this protein contains both the SUMO-interacting motif (SIM) and RING finger domains, it links sumoylation to ubiquitination.Immunogensynthetic peptide corresponding to a sequence at the C terminal region of human RNF4
Biochem/physiol Actions
RNF4 (ring finger protein 4) plays a wide variety of physiological roles, such as targeting of proteins for degradation, including promyelocytic leukemia protein (PML), CENP-I (centromere protein I), hypoxia-inducible factor (HIF), PARP-1 (poly [ADP-ribose] polymerase 1) etc. It also induces the base excision repair machinery, thus controlling DNA methylation. It is involved in DNA demethylation therefore, promoting gene transcription. RNF4 acts upon the oncogenic protein, Tax, of human T-cell leukemia virus type 1 (HTLV-1), and it regulates its nucleo-cytoplasmic localization. Arsenic trioxide (ATO) facilitates the sumoylation of promyelocytic leukemia (PML) bodies, thus, increasing their RNF4-mediated SUMO-dependent ubiquitylation and degradation. RNF4 functions as a co-regulator of androgen receptor-dependent transcription and is expressed in mice testis. Therefore, it might be involved in spermatid maturation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone RN.4, monoclonal form buffered aqueous solution species reactivity human packaging antibody small pack of 25 µL concentration ~1 mg/mL application(s) flow cytometry: 1-3 µg/test using HeLa cells","immunoblotting: 2-4 µg/mL using whole extracts of HEK-293 cells.","immunofluorescence: 0.25-0.5 µg/mL using HeLa cells. isotype IgG1 UniProt accession no. P78317, shipped in dry ice storage temp. 20°C Gene Information human ... RNF4(6047)
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Monoclonal Anti-RNF40 antibody produced in mouse
MDL number: MFCD08705600 NACRES: NA.41
Кат. номер R9029-200UL R9029-25UL Immunogensynthetic peptide corresponding to amino acids 548-564 of human RNF40.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone KA7-27, monoclonal form buffered aqueous solution mol wt antigen ~120 kDa species reactivity monkey, human packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) indirect ELISA: suitable","western blot: 1-2 µg/mL using HEK-293T cell extract isotype IgG1 conjugate unconjugated UniProt accession no. O75150, shipped in dry ice storage temp. 20°C Gene Information human ... RNF40(9810)
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Monoclonal Anti-Seladin-1 antibody produced in mouse
MDL number: MFCD09265376 NACRES: NA.41
Кат. номер S4697-25UL S4697-200UL General descriptionMonoclonal Anti-Seladin-1 (mouse IgG2b isotype) is derived from the hybridoma SL-14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human seladin-1.Seladin-1 is a human homolog of the Diminuto/Dwarf1 gene described in plants and C elegans. Seladin-1 (selective alzheimer′s disease indicator-1, 60 kDa) is encoded by the DHCR24 (3-β-hydroxysterol-Δ-24- reductase) gene. Seladin-1 is mainly located in the endoplasmic reticulum.Immunogensynthetic peptide corresponding to amino acids 505-516 of human seladin-1, conjugated to KLH.ApplicationMonoclonal Anti-Seladin-1 antibody produced in mouse has been used in:- enzyme linked immunosorbent assay (ELISA),
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Seladin-1 (selective Alzheimer′s disease indicator-1, 60 kDa), is an anti-apoptotic protein, found to be down regulated in large pyramidal neurons in brain regions affected by Alzheimer′s disease (AD). It is a flavin-adenine-dinucleotide (FAD)-dependent oxidoreductase, involved in cholesterol homeostasis. It catalyzes the conversion of desmosterol to cholesterol. It prevents apoptosis via inhibition of caspase-3, a key mediator of the apoptotic cascade, suggesting that seladin-1 may be involved in the regulation of cell survival and death. Seladin-1 has been shown to function as a key mediator of Ras-induced senescence in mouse and human fibroblasts. In response to oncogenic and oxidative stress, seladin-1 binds to p53 and displaces the E3 ubiquitin ligase Mdm2 from p53, resulting in accumulation of p53. The expression of seladin-1 has been reported to be up-regulated in adrenocortical adenomas and in some tumors.
Physical form
Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone SL-14, monoclonal form buffered aqueous solution mol wt antigen ~60 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunocytochemistry: suitable","indirect ELISA: suitable","western blot: 1-2 µg/mL using extracts of HEK-293T cells expressing human seladin-1 isotype IgG2b conjugate unconjugated UniProt accession no. Q15392, shipped in dry ice storage temp. 20°C Gene Information human ... DHCR24(1718)
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Monoclonal Anti-SNX6 antibody produced in mouse
NACRES: NA.41
Кат. номер S6324-25UL S6324-200UL General descriptionMonoclonal Anti-SNX6 (mouse IgA isotype) is derived from the hybridoma SNX6-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.
SNX6 (sorting nexin 6) is a BACE1 (Β-site APP cleaving enzyme-1)-associated protein and a constituent of retromer. It belongs to the sorting nexin (SNX) family that has a PX (Phox homology) domain at its amino terminus and a BAR (Bin/amphiphysin/Rvc) domain at its carboxyl region. It is located on chromosome 14q13.ApplicationMonoclonal Anti-SNX6 antibody produced in mouse has been used in immunoblotting and ELISA.
Biochem/physiol Actions
SNX6 (sorting nexin 6) helps in retrograde trafficking of the cation-independent mannose-6-phosphate receptor (CI-MPR) and sortilin. Interaction of SNX6 with breast cancer metastasis suppressor-1 induces transcriptional repression. It helps in the synthesis of lamin A and its transportation to the nuclear envelope .
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form ascites fluid antibody product type primary antibodies clone SNX6-76, monoclonal form buffered aqueous solution mol wt antigen ~47 kDa species reactivity rat, human, bovine, mouse packaging antibody small pack of 25 µL application(s) indirect ELISA: suitable","western blot: 1:2,500-1:5,000 using total extracts of A549 cells isotype IgA conjugate unconjugated UniProt accession no. Q9UNH7, shipped in dry ice storage temp. 20°C Gene Information human ... SNX6(58533)
mouse ... Snx6(72183)
rat ... Snx6(362738)
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Monoclonal Anti-TIGAR antibody produced in mouse
NACRES: NA.41
Кат. номер T8828-25UL T8828-200UL General descriptionTP53-induced glycolysis and apoptosis regulator (TIGAR) is a p53 target gene mapped to human chromosome 12p13-3. The gene contains six exons and it is characterized with two possible p53 binding sites, one upstream of the first exon (BS1) and one within the first intron (BS2).
Monoclonal Anti-TIGAR (mouse IgG2b isotype) is derived from the hybridoma 9C10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to a fragment in exon 6-encoding region of human TIGAR . TIGAR expression was found to correlate with fludarabine sensitivity in chronic lymphocytic leukemia (CLL) cells.ApplicationMonoclonal Anti-TIGAR antibody produced in mouse has been used in:- enzyme linked immuno sorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
Biochem/physiol Actions
TP53-induced glycolysis and apoptosis regulator (TIGAR) inhibits glycolysis by decreasing the fructose-2, 6-bisphosphate levels in cells. This p53 induced protein also plays a vital role in regulation of intracellular reactive oxygen species (ROS) levels. TIGAR expression is associated with oncogenesis and development of several human cancers. Overexpression of TIGAR is observed in colorectal cancer patients. Thus, TIGAR expression might function as a potential biomarker for diagnosis of colorectal cancer and it can act as a target for developing therapeutics for the treatment of colorectal cancer. Nullified expression of TIGAR, increases the radiosensitivity of tumor cells and also enhances the autophagy activity in cells with nutrient starvation or metabolic stress. TIGAR increases cancer cell survival rate in response to tumor chemotherapy by inhibiting both apoptosis and autophagy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 9C10, monoclonal form buffered aqueous solution mol wt antigen ~30 kDa species reactivity monkey, human packaging antibody small pack of 25 μL concentration ~1.0 mg/mL application(s) immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using HeLa nuclear extract isotype IgG2b conjugate unconjugated shipped in dry ice storage temp. −20°C Gene Information human ... C12orf5(57103)
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Monoclonal Anti-TRF1 antibody produced in mouse
MDL number: MFCD03792434 NACRES: NA.41
Кат. номер T1948-25UL T1948-100UL T1948-200UL General descriptionMonoclonal Anti-TRF1 (mouse IgG1 isotype) is derived from the TRF-78 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a human TRF1 protein produced in baculovirus. TRF1 and TRF2 (TTAGGG repeat binding factors) are two major proteins that bind to human telomers. TRF1 has a DNA binding domain with high homology to the Myb family of transcription factors. Unlike the Myb family that contains only one DNA binding motif, TRF1 has multiple of this motif.
Specificity
Monoclonal Anti-TRF1 recognizes human TRF1.Immunogenhuman TRF1 protein produced in baculovirus.ApplicationMonoclonal Anti-TRF1 antibody produced in mouse has been used in Western blotting and enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
TRF1 (TTAGGG repeat binding factor 1) has a negative effect on the length of the telomer. Overexpression of TRF1 in cancer cells that contain telomerase activity, causes the shortening of the length of their telomers. While inhibition of TRF1 causes the elongation of telomers. It was shown that the level of TRF1 in the cells does not affect the expression of the telomerase protein. This suggests that TRF1 may act directly on the activity of the telomerase protein. Tankyrase is a protein that interacts with TRF1 and its C-terminal region is homologous to poly (adenosine dinucleotide phosphate (ADP)-ribose) polymerase (PARP). In response to DNA damage, the PARP protein mediates ADP-ribose polymers of protein acceptors. In vitro studies have shown that tankyrase is responsible for that polyribosylation of TRF1, which in turn abolishes its ability to bind telomers.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone TRF-78, monoclonal form buffered aqueous solution mol wt antigen ~70 kDa by SDS-PAGE species reactivity human packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) indirect ELISA: suitable","microarray: suitable","western blot: 2-4 µg/mL using HeLa nuclear extract isotype IgG1 conjugate unconjugated UniProt accession no. P54274, shipped in dry ice storage temp. 20°C Gene Information human ... TERF1(7013)
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Monoclonal Anti-Tumor Necrosis Factor- antibody produced in hamster
MDL number: MFCD01632994 NACRES: NA.41
Кат. номер T2824-25UL T2824-200UL General descriptionMonoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.
Specificity
Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.Immunogenpurified recombinant mouse TNF-α.ApplicationMonoclonal Anti-Tumor Necrosis Factor- antibody produced in hamster has been used in:- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunoprecipitation
- neutralization
Packaging
200 μL in glass insert
Biochem/physiol Actions
TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.
Physical form
Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source hamster antibody form purified immunoglobulin antibody product type primary antibodies clone TN3-19.12, monoclonal form buffered aqueous solution species reactivity rat, mouse packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","neutralization: suitable","western blot: 0.25-0.5 µg/mL using recombinant mouse TNF- conjugate unconjugated UniProt accession no. P16599, shipped in dry ice storage temp. 20°C Gene Information mouse ... Tnf(21926)
rat ... Tnf(24835)
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Monoclonal Anti-U2AF65 antibody produced in mouse
MDL number: MFCD03456068 NACRES: NA.41
Кат. номер U4758-25UL U4758-100UL U4758-200UL General descriptionMonoclonal Anti-U2AF65 (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF65. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF65) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF65kDa subunit is a non-snRNP protein component of spliceosome complexes.Immunogenrecombinant human U2AF65.ApplicationMonoclonal Anti-U2AF65 antibody produced in mouse has been used in:- RNA immunoprecipitation
- immunoblotting
- chromatin immunoprecipitation (ChIP)
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunocytochemistry
- immunoelectron microscopy
Biochem/physiol Actions
U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone MC3, monoclonal form buffered aqueous solution mol wt antigen ~65 kDa species reactivity human, Xenopus, rat, mouse packaging antibody small pack of 25 µL application(s) electron microscopy: suitable","immunocytochemistry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.1-0.2 µg/mL using total cell extract from HeLa cells isotype IgG2b conjugate unconjugated UniProt accession no. P26368, shipped in dry ice storage temp. 20°C Gene Information human ... U2AF2(11338)
mouse ... U2af2(22185)
rat ... U2af2(308335)
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Monoclonal Anti-USP7 antibody produced in rat
NACRES: NA.41
Кат. номер SAB4200042-25UL SAB4200042-200UL General descriptionMonoclonal Anti-USP7 (rat IgG2a isotype) is derived from the hybridoma USP 3D8 produced by the fusion of mouse myeloma cells (P3X63 AG 8.653) and splenocytes from rat immunized with a peptide.
Ubiquitin specific peptidase 7 (USP7) is a kind of deubiquitylating (DUB) enzyme, which regulates tumor protein p53-mouse double minute 2 homolog (p53-mdm2) pathway. USP7 gene is located on human chromosome 16p13.2.
Specificity
Monoclonal Anti-USP7 recognizes human and rat USP7.ApplicationMonoclonal Anti-USP7 antibody produced in rat may be used in:- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Overexpression of the USP7 gene results in p53-mediated apoptosis. Ablation of the USP7 gene expression results in p53 accumulation as opposed to the expected destabilization of p53. This effect has been traced to the ability of USP7 to stabilize mouse double minute 2 homolog (MDM2), a ubiquitin ligase that promotes the degradation of p53. Therefore, USP7 appears to play multiple roles in regulating the p53-MDM2 pathway and in maintaining steady-state levels of p53 in the cell. USP7 is considered for treating hematopoietic tumors due to its role in p53 regulation.
Ubiquitin specific peptidase 7 (USP7) promotes oncogenesis and recognizes specific inhibitors. USP7 is overexpressed in hepatocellular carcinoma (HCC) tissues, which contributes to tumor invasion and proliferation. USP7 is important for osteogenic differentiation of human adipose-derived stem cells (hASCs). It is used for hASC based stem cell therapy and bone tissue engineering. USP7 plays complex roles in DNA regulation, p53 stress response and endosomal protein recycling.
Target description
USP7 is ubiquitin specific peptidase 7 (herpes virus-associated). It is associated with induction of apoptosis, interspecies interaction between organisms, protein deubiquitination, regulation of protein stability, and ubiquitin-dependent protein cataboli
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone USP 3D8, monoclonal form buffered aqueous solution mol wt antigen ~130 kDa species reactivity rat, human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: 2-4 µg/mL using HeLa cell extracts isotype IgG2a conjugate unconjugated UniProt accession no. Q93009, shipped in dry ice storage temp. 20°C Gene Information human ... USP7(7874)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-ZAP-70 antibody produced in mouse
MDL number: MFCD00803577 NACRES: NA.44
Кат. номер Z0627-25UL Z0627-200UL General descriptionMonoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells.ImmunogenThe immunogen sequence is conjugated to KLH.
peptide corresponding to amino acids 282-307 of human ZAP-70.ApplicationMonoclonal Anti-ZAP-70 antibody has been used in- enzyme linked immunosorbent assay (ELISA)
- immunoblottin
- flow cytometry
- immunoprecipitation
- immunocytochemistry
Monoclonal Anti-ZAP-70 antibody is suitable for use in western blot (0.5-1.0 μg/mL using Jurkat cell extract), flow cytometry, immunocytochemistry, immunoprecipitation, microarray and indirect ELISA.
Biochem/physiol Actions
Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID).
ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 1E7.2, monoclonal form buffered aqueous solution mol wt antigen ~70 kDa species reactivity mouse, human packaging antibody small pack of 25 µL concentration ~1.5 mg/mL application(s) flow cytometry: suitable","immunocytochemistry: suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.5-1.0 µg/mL using Jurkat cell extract isotype IgG1 conjugate unconjugated UniProt accession no. P43403, shipped in dry ice storage temp. 20°C Gene Information human ... ZAP70(7535)
mouse ... Zap70(22637)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mouse IgG1 Isotype Control (MOPC-21) ColorWheel® Dye-Ready mAb CWA-1013-25UL
Specificity
Clone MOPC-21 is an isotype control antibody for Mouse IgG1.ApplicationQuality Control Testing:
Evaluated by Flow Cytometry in Protein A / G beads.
Flow Cytometry Analysis: Mouse IgG1 Isotype Control (MOPC-21) Colorwheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Mouse IgG1 Isotype Control (MOPC-21) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Mouse IgG1 Isotype Control, clone MOPC-21 is suitable for use an isotype-matched control for Mouse IgG1. It has unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between a mouse IgG1 antibody and the sample under defined experimental conditions.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.Legal InformationColorWheel is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 200, biological source mouse antibody form purified antibody antibody product type primary antibodies clone MOPC-21, monoclonal product line ColorWheel® form lyophilized species reactivity mouse packaging antibody small pack of 25 µL application(s) flow cytometry: suitable isotype IgG1 shipped in ambient storage temp. 2-8°C Узнать цену -
Mouse IgG2b Isotype Control (MPC-11) ColorWheel® Dye-Ready mAb CWA-1010-25UL
Specificity
Clone MPC-11 is an isotype control antibody for mouse IgG2b.ApplicationMouse IgG2b Isotype Control (MPC-11) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Quality Control Testing:
Evaluated by Flow Cytometry with Protein A/G beads.
Mouse IgG2b Isotype Control (MPC-11) Colorwheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.
Target description
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Mouse IgG2b Isotype Control, clone MPC-11 is suitable for use an isotype-matched control for murine IgG2b. It has unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between a mouse IgG2b antibody and the sample under defined experimental conditions.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.Legal InformationColorWheel is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 200, biological source mouse antibody form purified antibody antibody product type primary antibodies clone MPC-11, monoclonal product line ColorWheel® form lyophilized species reactivity mouse packaging antibody small pack of 25 µL application(s) flow cytometry: suitable isotype IgG2b shipped in ambient storage temp. 2-8°C Узнать цену -
Mouse IgM Isotype Control (11E10) ColorWheel® Dye-Ready mAb CWA-1017-25UL
General descriptionColorwheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Specificity
Clone 11E10 is an isotype control monoclonal antibody for Mouse igM.ApplicationMouse IgM Isotype Control (11E10) Colorwheel® Dye-Ready mAb, Cat. No. CWA-1017, is a mouse monoclonal isotype control antibody that is tested for use in Flow Cytometry.
Quality Control Testing:
Evaluated by Flow Cytometry with Protein A/G beads.
Mouse IgM Isotype Control (11E10) Colorwheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.
Target description
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Mouse IgM Isotype Control, clone 11E10 is suitable for use an isotype-matched control for Mouse IgM. It can be used for evaluation of background staining caused by non-specific interactions between a mouse IgM antibody and the sample under defined experimental conditions.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.Legal InformationColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200, biological source mouse antibody form purified antibody antibody product type primary antibodies clone 11E10, monoclonal product line ColorWheel® form lyophilized packaging antibody small pack of 25 µL application(s) flow cytometry: suitable isotype IgM shipped in ambient storage temp. 2-8°C Узнать цену -
Rabbit IgG Isotype Control ColorWheel® Dye-Ready pAb CWA-1053-25UL
Specificity
This rabbit polyclonal antibody can be used as an isotype control for Rabbit IgG.ApplicationQuality Control Testing:
Evaluated by Flow Cytometry with Protein A/G beads.
Rabbit IgG Isotype Control Colorwheel® Dye-Ready pAb was tested by flow cytometry using Protein A/G beads.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Rabbit IgG Isotype Control ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Rabbit IgG Isotype Control is suitable for use an isotype-matched control for Rabbit IgG and can be used for evaluation of background staining caused by non-specific interactions between a rabbit IgG antibody and the sample under defined experimental conditions.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.Legal InformationColorWheel is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыbiological source rabbit Quality Level 200, antibody form purified antibody antibody product type primary antibodies clone N/A, monoclonal product line ColorWheel® form lyophilized packaging antibody small pack of 25 µL application(s) flow cytometry: suitable isotype IgG shipped in ambient storage temp. 2-8°C Узнать цену -
Rat IgG1 Isotype Control (HRPN) ColorWheel® Dye-Ready mAb CWA-1011-25UL
Specificity
Clone HRPN is an isotype control antibody for rat IgG1.Rat IgG1 Isotype Control (HRPN) Colorwheel® Dye-Ready mAbApplicationQuality Control Testing:
Evaluated by Flow Cytometry in Protein A/G beads.
Rat IgG1 Isotype Control (HRPN) Colorwheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.
Rat IgG1 Isotype Control (HRPN) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Rat IgG1 Isotype Control, clone HRPN is suitable for use as an isotype-matched control for rat IgG1. It has an unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between a rat IgG1 and the sample under defined experimental conditions.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.Legal InformationColorWheel is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыbiological source rat Quality Level 200, antibody form purified antibody antibody product type primary antibodies clone HRPN, monoclonal product line ColorWheel® form lyophilized species reactivity rat packaging antibody small pack of 25 µL application(s) flow cytometry: suitable isotype IgG1 shipped in ambient storage temp. 2-8°C Узнать цену -
Rat IgG2b Isotype Control (LTF-2) ColorWheel® Dye-Ready mAb CWA-1012-25UL
Specificity
Clone LTF-2 is an isotype control antibody for rat IgG2b.ApplicationQuality Control Testing:
Evaluated by Flow Cytometry in Protein A/G beads.
Rat IgG2b Isotype Control (LTF-2) Colorwheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.
Rat IgG2b Isotype Control (LTF-2) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Rat IgG2b Isotype Control, clone LTF-2 is suitable for use as an isotype-matched control for rat IgG2b. It has an unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between a rat IgG2b and the sample under defined experimental conditions.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.Legal InformationColorWheel is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыbiological source rat Quality Level 200, antibody form purified antibody antibody product type primary antibodies clone LTF-2, monoclonal product line ColorWheel® form lyophilized species reactivity rat packaging antibody small pack of 25 µL application(s) flow cytometry: suitable isotype IgG2b shipped in ambient storage temp. 2-8°C Узнать цену
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Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
