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Small Pack Antibodies
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Anti-SREBP1 Antibody, clone 2121
eCl@ss: 32160702 NACRES: NA.41
Кат. номер 4-469-25UL 4-469 4-469-100UL
Specificity
Reacts with the basic helix-loop-helix leucine zipper (bHLH-ZIP) of SREBP.ImmunogenRecombinant His-tagged human SREBP-1a protein encompassing amino acids 301-407.ApplicationAnti-SREBP1 Antibody, clone 2121 is a Mouse Monoclonal Antibody for detection of SREBP1 also known as sterol regulatory element binding transcription factor 1 & has been validated in WB.
Quality
Routinely evaluated by immunoblot.
Target description
126 kDa
Physical form
100 μL (1 mg/mL) of protein A purified mouse monoclonal IgG1 in PBS with 0.1% sodium azide.
Format: Purified
Storage and Stability
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.Other Notesfeature_concentration_valuePlease refer to lot specific datasheet.Legal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 100 biological source mouse antibody form purified antibody antibody product type primary antibodies clone 2121, monoclonal species reactivity human packaging antibody small pack of 25 µL Торговая марка Upstate® application(s) western blot: suitable isotype IgG1 NCBI accession no. NM_001005291.1,","NM_004176.3, UniProt accession no. P36956, shipped in dry ice -
Anti-SRP68 antibody produced in rabbit
Human Protein Atlas Number: HPA023303 Human Protein Atlas characterization data
Кат. номер HPA023303-100UL HPA023303-25UL General descriptionThe gene SRP68 (signal recognition particle 68) is mapped to human chromosome 17q25.ImmunogenSignal recognition particle 68 kDa protein recombinant protein epitope signature tag (PrEST)ApplicationAll Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org),and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols, and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige,.
Biochem/physiol Actions
SRP68 (signal recognition particle 68) is a part of the SRP ribonucleoprotein complex. The complex is crucial for the co-translational transfer of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP68 interacts with SRP72 and the heterodimer is needed for SRP-associated transfer of proteins to the ER. In addition, the heterodimer also binds to the chromatin and participates in transcriptional regulation.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
LinkageCorresponding Antigen APREST75779,.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azideLegal InformationPrestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous glycerol solution species reactivity human packaging antibody small pack of 25 µL application(s) immunoblotting: 0.04-0.4 µg/mL","immunofluorescence: 0.25-2 µg/mL","immunohistochemistry: 1:20-1:50 immunogen sequence DAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEAFNKCKTIYEKLASAFTEEQAVLYNQRVEEISPNIRYCAYNIGDQSAINELMQMRLRSGGTEGLLAEKLEALITQTRAKQAATMSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIV conjugate unconjugated UniProt accession no. Q9UHB9, shipped in wet ice storage temp. 20°C Gene Information human ... SRP68(6730)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-ST8SIA1 Antibody, clone HIC0-3C5
eCl@ss: 32160702 NACRES: NA.41
Кат. номер MABS1997-100UG MABS1997-25UG General descriptionAlpha-N-acetylneuraminide alpha-2,8-sialyltransferase (UniProt: Q92185; also known as EC: 2.4.99.8, Alpha-2,8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI) I encoded by the ST8SIA1 (also known as SIAT8, SIAT8A) gene (Gene ID: 6489) in human. SIAT8-A is a single-pass type II membrane protein that is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. It is involved in the production of gangliosides GD3 and GT3 from GM3. It catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3 in a successive manner. It is also involved in the pathway protein glycosylation. SIAT8-A contains a cytoplasmic domain (aa 1-29); a transmembrane domain (aa 30-48); and a lumenal domain (aa 49-356). Two isoforms of SIAT8-A have been described that are produced by alternative splicing. (Ref.: Dorrell, C., et al. (2016). Nat. Commun. 7; 11756).
Specificity
Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).ImmunogenTrypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.ApplicationFlow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Research Category
Signaling
Anti-ST8SIA1, clone HIC0-3C5, Cat. No. MABS1997, is a mouse monoclonal antibody that detects Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase and has been tested for use in Flow Cytometry, Immunoflurescence, and Immunohistochemistry.
Quality
Evaluated by Immunohistochemistry in human pancreatic tissue sections.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected ST8SIA1 in human pancreatic tissue sections.
Target description
40.52 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Format: Purified
Purified by ion-exchange chromatography
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source mouse antibody form purified antibody antibody product type primary antibodies clone HIC0-3C5, monoclonal species reactivity human packaging antibody small pack of 25 µg application(s) flow cytometry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable isotype IgM NCBI accession no. NP_003025.1, UniProt accession no. Q92185, Gene Information human ... ST8SIA1(6489) -
Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480
eCl@ss: 32160702
Кат. номер MAB4301 MAB4301-25UG MAB4301-100UG General descriptionSSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Specificity
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).ImmunogenF9 tetracarcinoma stem cells (X-irradiated).ApplicationResearch Sub Category
Pluripotent & Early Differentiation
Neural Stem Cells
Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Immunohistochemistry:
A previous lot of this antibody was tested on IH.
Immunoprecipitation:
A previous lot of this antibody was tested on IP.
Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.
Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.
Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Quality
Tested
Target description
220 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
Ammonium Sulfate Precipitation
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
P19 mouse embryonic carcinoma cellsOther NotesConcentration: Please refer to the Certificate of Analysis for the lot-specific concentration.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 biological source mouse antibody form saturated ammonium sulfate (SAS) precipitated antibody product type primary antibodies clone MC-480, monoclonal species reactivity rat, human, mouse packaging antibody small pack of 25 µg Торговая марка Chemicon® application(s) flow cytometry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable input sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)isotype IgM NCBI accession no. NM_002033.2, UniProt accession no. P22083, shipped in ambient storage temp. 2-8°C Gene Information human ... FUT4(2526) -
Anti-Staphylococcal -Toxin antibody produced in rabbit
MDL number: MFCD00162866 NACRES: NA.46
Кат. номер S7531-25UL S7531-1ML General descriptionStaphylococcal toxin, a water soluble 33kD single polypeptide, is a cytotoxic agent secreted by Staphylococcus aureus. This protein has membrane damaging properties and accounts for erythrocyte lysis. Hence it is also referred to as lethal hemolytic toxin or -hemolysin . Anti-staphylococcal -toxin antibody can be used for studies of the toxin-membrane interaction. Rabbit anti-staphylococcal -toxin antibody reacts specifically with staphylococcal -toxin but not with staphylococcal enterotoxin A, cholera toxin or pseudomonas exotoxin A.Immunogen-toxin (-hemolysin) from Staphylococcus aureusApplicationAnti-staphylococcal α-toxin antibody can be used in indirect ELISA (1:50,000) and dot blot immunoassay (1: 20,000).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper),
Quality
The antiserum has been treated to remove lipoproteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 200, antibody form whole antiserum antibody product type primary antibodies clone polyclonal mol wt antigen 33 kDa contains 15 mM sodium azide species reactivity Staphylococcus aureus packaging antibody small pack of 25 µL application(s) dot blot: 1:20,000, indirect ELISA: 1:50,000 conjugate unconjugated shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable -
Anti-STRA6
eCl@ss: 32160702 NACRES: NA.41
Кат. номер ABN1662 ABN1662-25UL General descriptionStimulated by retinoic acid gene 6 protein (UniProt: O70491; also known as Retinoic acid-responsive protein, STRA6) is encoded by the Stra6 gene (Gene ID: 20897) in murine species. STRA6 is a multi-pass membrane protein that acts as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). It has one intramembrane and nine transmembrane helices in a dimeric assembly. It acts by removing retinol from RBP/RBP4 and transports it across the plasma membrane, where it can be metabolized. This mechanism does not depend on endocytosis. Binds to RBP/RBP4 with high affinity. Increases cellular retinol uptake from the retinol-RBP complex. STRA6 is widely expressed in the embryo and it in adults it is expressed choroid plexus and the brain microvascular cells that form the blood-organ barrier. It is also expressed in the retinal pigment epithelium, seminiferous epithelium in testis, the yolk sac, and in chorioallantoic placenta. Its expression in Sertoli cells depends on the stage of the spermatogenic cycle. STRA6 can be induced by retinoic acid and it is shown to be synergistically up-regulated by Wnt1 and retinoid in mammary epithelial cells. Mutations in Stra6 gene have been linked to the Matthew-Wood syndrome that is characterized by pulmonary agenesis/hypoplasia; microphthalmia/anophthalmia; and congenital cardiac, digestive, and urogenital malformations. (Ref.: Sadowski, S et al. (2017). Birth Defects Res. 109(4); 251-253; Chen, Y et al. (2016). Science 353 (6302), aad8266).
Specificity
This rabbit polyclonal antibody detects murine Stimulated by retinoic acid gene 6 protein (STRA6). it targets an epitope within 109 amino acids from the C-terminal region.ImmunogenHis-tggged recombinant fragment corresponding to 109 amino acids from the C-terminal region of murine Stimulated by retinoic acid gene 6 (STRA6) protein.
Epitope: C-terminusApplicationImmunoprecipitation Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy).
Western Blotting Analysis: A representative lot detected STRA6 in Western Blotting applications (Berry, D.C., et. al. (2012). Mol Cell Biol. 32(19):3851-9).
Western Blotting Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy).
Flow Cytometry Analysis: A 1:100 dilution from a representative lot detected STRA6 in HT-29 cells.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected STRA6 in mouse testis and human liver cancer tissues.
Research Category
Neuroscience
Anti-STRA6, Cat. No. ABN1662, is a highly specific rabbit polyclonal antibody that targets Stimulated by retinoic acid gene 6 protein (STRA6) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Quality
Evaluated by Western Blotting in NIH3T3/L1Adipocytes.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STRA6 in NIH3T3/L1 Adipocytes.
Target description
~90 kDa observed; 73.77 kda calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form unpurified antibody product type primary antibodies clone polyclonal species reactivity mouse packaging antibody small pack of 25 µL application(s) flow cytometry: suitable","immunohistochemistry: suitable (paraffin)","immunoprecipitation (IP): suitable","western blot: suitable isotype IgG NCBI accession no. NP_033317, UniProt accession no. O70491, shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-Strep-Tag Antibody, clone C23.21
Кат. номер MAC143-25UL MAC143-100UL General descriptionStrep-Tag is a short amino acid-peptide sequence that exhibits specific binding to streptavidin. It is shown to reversibly occupy the same pocket where biotin is normally complexed. Hence, it is highly desirable for applications involving where efficient purification of fusion proteins. Use of this tag allows for easier detection of fusion proteins in various immunochemical assays. Strep-Tag is shown to be resistant to the action of proteases and can also be used in the presence of mild detergents. Clone C23.21 recognizes fusion proteins with Strep-Tag peptide sequence WSHPQFEK with picomolar affinity. It recognizes both the single and the double Strep-Tag sequence.
Specificity
Clone C23.21 is a mouse monoclonal antibody that detects Strep-Tagged proteins. It binds to WSHPQFEQ sequence with picomolar affinity.ImmunogenRecombinant ectodomain of glycoprotein E2 of GB virus (GBV-B), containing a double Strep-Tag (two Strep-Tags separated by a linker peptide)ApplicationAnti-Strep-Tag, clone C23.21, Cat. No. MAC143, is a mouse monoclonal antibody that detects Strep-Tagged proteins and is tested for use in Immunohistochemistry, Surface plasmon resonance, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting with Strep-Tagged VHH 9.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Strep-Tagged VHH9.
Tested Applications
Surface plasmon resonance: A representative lot detected Strep-Tag in Surface plasmon resonance application. (Meola, A., et al. (2015). J Virol. 89(4):2170-81).
Western Blotting Analysis: A representative lot detected Strep-Tag in human brain extract and GFAP in Western Blotting applications. (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Immunohistochemistry Applications: A representative lot detected Strep-Tag in mouse brain tissue sections in Immunohistochemistry applications (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.Other NotesConcentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified antibody antibody product type primary antibodies clone C23.21, monoclonal mol wt observed mol wt ~17 kDa purified by using protein G species reactivity all packaging antibody small pack of 100 µL application(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable isotype IgG1 epitope sequence Unknown conjugate unconjugated UniProt accession no. N/A, shipped in 2-8°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-STRN3
eCl@ss: 32160702 NACRES: NA.41
Кат. номер ABS2099 ABS2099-25UG General descriptionStriatin-3 (UniProt: Q13033; also known as Cell cycle autoantigen SG2NA, S/G2 antigen) is encoded by the STRN3 (also known as GS2NA, SG2NA) gene (Gene ID: 29966) in human. Striatin-3 is a calmodulin-binding protein that bonds calmodulin a calcium dependent manner and may function as scaffolding or signaling protein. Two isoforms of Striatin-3 have been reported that are produced by alternative splicing. Its calmoduln-binding region is located within amino acids 166-183. It also contains a caveolin-binding region (aa 71-79), six WD repeats, and a coiled coil region (aa 77-136). The coiled coil domain and the PP2A subunit can form a stable core complex with a 2:2 stoichiometry. The coiled coil domain of striatin-3 is a novel type of PP2A regulatory subunit that functions as a scaffold for the assembly of the STRIPAK complex. PP2A and certain kinases, such as germinal central kinase III (GCKIII) interact with striatin to form striatin-interacting phosphatase and kinase (STRIPAK) complex that are known to regulate multiple cellular events. Wild-type striatin-3 is shown to strongly suppress apoptosis in Jurkat cells induced by the GCKIII kinase MST3. However, mutant forms of striatin-3 fail to exhibit this effect. (Ref.: Lu, Q., et al. (2004). Proc. Natl. Acad. Sci. USA 101(49); 17126-17131; Chen, C., et al. (2014). J. Biol. Chem. 289(14); 9651-61).
Specificity
This rabbit polyclonal antibody detect Striatin-3 in human and murine cells. It targets an epitope within 17 amino acids from the N-terminal half.ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Striatin-3. The immunogen sequence is conserved in both alpha and beta isoforms.
Epitope: unknownApplicationImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STRN3 in HeLa cells.
Research Category
Signaling
Anti-STRN3, Cat. No. ABS2099, is a rabbit polyclonal antibody that detects Striatin-3 and has been tested for use in Immunocytochemistry and Western Blotting.
Quality
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected STRN3 in 10 µg of NIH3T3 cell lysate.
Target description
~87 kDa observed; 87.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal species reactivity human, mouse packaging antibody small pack of 25 µg application(s) immunocytochemistry: suitable","western blot: suitable isotype IgG NCBI accession no. NP_001077362, UniProt accession no. Q13033, shipped in ambient Gene Information human ... STRN3(29966)
Safety InformationFlash Point F does not flash Flash Point C does not flash -
Anti-STT3A antibody produced in rabbit
Human Protein Atlas Number: HPA030735 Human Protein Atlas characterization data
Кат. номер HPA030735-100UL HPA030735-25UL ImmunogenSTT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) recombinant protein epitope signature tag (PrEST)ApplicationAll Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org),and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols, and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige,.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
LinkageCorresponding Antigen APREST77889,.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.Legal InformationPrestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous glycerol solution species reactivity human packaging antibody small pack of 25 µL application(s) immunohistochemistry: 1:20-1:50 immunogen sequence VRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKR conjugate unconjugated UniProt accession no. P46977, shipped in wet ice storage temp. 20°C Gene Information human ... STT3A(3703)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-Substance P Receptor antibody produced in rabbit
MDL number: MFCD01634202 NACRES: NA.41
Кат. номер S8305-.2ML S8305-25UL General descriptionSubstance P receptor (SPR), also known as neurokinin receptor 1 (NK-1 R), is a member of the G-protein-coupled class of seven transmembrane domain receptors. SPR gene with five exons, spanning 45-60kb on genomic DNA, is mapped to human chromosome 2. SPR is highly expressed in central nervous system.Immunogensynthetic peptide corresponding to the C-terminal of NK1R of rat origin (amino acids 393-407). This sequence is highly conserved in mouse, guinea pig, and human NK1R, but diverges in other tachykinin receptor subtypes NK2R and NK3R.ApplicationAnti-substance P receptor antibody produced in rabbit has been used in immunohistochemistry.
Biochem/physiol Actions
Substance P (SP) exhibits pro-inflammatory, endocrine, neuromodulatory and trophic activities. Substance P receptor (SPR) upon binding to its ligand SP, facilitates extravasation of granulocytes and in inflammation and tissue derangement. In addition, activation of this receptor also enhances metabolism of phosphoinositol, leading to synthesis of inositol-1,4,5-trisphosphate and diacylglycerol which results in release of Ca2+ from the endoplasmic reticulum and activation of protein kinase C. SPR along with its ligand stimulates tumoral angiogenesis and cell proliferation in a variety of cancer types. Inhibition of substance P receptor retards the growth of neuroblastoma (NB) cell lines. Thus, SPR might act as a potential therapeutic target for a large variety of patients with neuroblastoma. SP-SPR complex is also implicated in the regulation of the pain transmission.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 200 antibody form IgG fraction of antiserum antibody product type primary antibodies clone polyclonal form buffered aqueous solution mol wt antigen 46 kDa species reactivity human, rat, guinea pig, mouse packaging antibody small pack of 25 µL application(s) immunohistochemistry (frozen sections): 1:5,000 using 4% paraformaldehyde (with picric acid/glutaraldehyde)-fixed, frozen, rat brain sections.","microarray: suitable","western blot (chemiluminescent): 1:2,000 using a rat brain membrane fraction extract conjugate unconjugated UniProt accession no. P25103, shipped in dry ice storage temp. 20°C Gene Information human ... TACR1(6869)
mouse ... Tacr1(21336)
rat ... Tacr1(24807)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable -
Anti-SUMO-1 Antibody, clone 21C7
eCl@ss: 32160702 NACRES: NA.41
Кат. номер MABS2071-100UG MABS2071-25UG General descriptionSmall ubiquitin-related modifier 1 (UniProt: P63165; also known as SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1) is encoded by the SUMO1 (also known as SMT3C, SMT3H3, UBL1, OK/SW-cl.43) gene (Gene ID: 7341) in human. SUMO-1 is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in many cellular processes, such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO-1 can also covalently attach to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO-1 chains are shown to be susceptible to polyubiquitination, which functions as a signal for proteasomal degradation of modified proteins. Two isoforms of SUMO-1 have been reported that are produced by alternative splicing. Mutations in SUMO1 gene are known to cause non-syndromic orofacial cleft 10, which is a birth defect consisting of cleft lips with or without cleft palate.
Specificity
Clone 21C7 detects SUMO-1 in multiple species. It targets an epitope with in 11 amino acids from the internal region.ImmunogenHis-tagged full length human recombinant Small ubiquitin-related modifier 1 (SUMO-1).ApplicationImmunohistochemistry Analysis: A representative lot detected SUMO-1 in Immunohistochemistry applications (Rogers, R.S., et. al. (2004). Chromosoma. 113(5):233-43; Costa, M.W., et. al. (2011). PLoA One. 6(9):e24812).
Immunocytochemistry Analysis: A representative lot detected SUMO-1 in Immunocytochemistry applications (Matunis, M.J., et. al. (1996). J Cell Biol. 135(6 Pt 1):1457-70; Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41).
Immunoprecipitation Analysis: A representative lot immunopprecipitated SUMO-1 in Immunoprecipitation applications (Costa, M.W., et. al. (2011). PLoA One. 6(9):e24812; Becker, J., et. al. (2013). Nat Struct Mol Biol. 20(4):525-31; Girach, F., et. al. (2013). Cell Rep. 5(5):1294-301; Chanda, A., et. al. (2017). PLoS One. 12(5):e0177639).
Western Blotting Analysis: A representative lot detected SUMO-1 in Western Blotting applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Matunis, M.J., et. al. (1996). J Cell Biol. 135(6 Pt 1):1457-70; Rogers, R.S., et. al. (2004). Chromosoma. 113(5):233-43; Costa, M.W., et. al. (2011). PLoA One. 6(9):e24812; Becker, J., et. al. (2013). Nat Struct Mol Biol. 20(4):525-31).
Immunofluorescence Analysis: A representative lot detected SUMO-1 in Immunofluorescence applications (Girach, F., et. al. (2013). Cell Rep. 5(5):1294-301; Rogers, R.S., et. al. (2004). Chromosoma. 113(5):233-43; Matunis, M.J., et. al. (1996). J Cell Biol. 135(6 Pt 1):1457-70).
Research Category
Signaling
Anti-SUMO-1, clone 21C7, Cat. No. MABS2071, is a mouse monoclonal antibody that detects Small ubiquitin-related modifier 1 (SUMO-1) and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
Quality
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected SUMO-1 in HCT116 cell lysate.
Target description
~17 kDa observed; 11.56 kDa calculated. SUMO-1/RanGAP1 band is observed AT ~ 75 kDa Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 21C7, monoclonal species reactivity rat, chicken, mouse, fish, zebrafish, human, Xenopus packaging antibody small pack of 25 µg application(s) immunocytochemistry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable isotype IgG2b NCBI accession no. NP_001005781, UniProt accession no. P63165, Gene Information human ... SUMO1(7341)
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-SUMO-2/3 Antibody, clone 8A2
eCl@ss: 32160702 NACRES: NA.41
Кат. номер MABS2039 MABS2039-25UG General descriptionSmall ubiquitin-related modifier 2 (UniProt P61956; also known as HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; also known as SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (also known as SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (also known as SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein post-translation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the de-SUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares only about 50% sequence homology with SUMO-2 and 3. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator. SUMO-2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Its covalent attachment to its substrate via an isopeptide bond requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Although SUMO-2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system, however, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus (aa 94-95; propeptide) have been cleaved off.
Specificity
Clone 8A2 detects SUMO-2/3 in human cells.ImmunogenGST-tagged full length recombinant human SUMO-2 protein.ApplicationAnti-SUMO-2/3, clone 8A2, Cat. No. MABS2039, is a mouse monoclonal antibody that detects SUMO-2 and SUMO-3 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected SUMO-2/3 in Western Blotting applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80).
Immunoprecipitation Analysis: A representative lot detected SUMO-2/3 in Immunoprecipitation applications (Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80).
Western Blotting Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cell lysate (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA).
Immunocytochemistry Analysis: A representative lot detected SUMO-2/3 in Immunocytochemistry applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Rao, H.B., et. al. (2017) Science. 355(6323):403-407).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected SUMO-2/3 in HeLa cell lysate.
Target description
~19 kDa observed; 10.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 8A2, monoclonal species reactivity human packaging antibody small pack of 25 µg application(s) immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable isotype IgG2b NCBI accession no. NP_008867.2,","NP_008868.3, UniProt accession no. P55854,","P61956, shipped in ambient Gene Information human ... SUMO2(6613)
Safety InformationFlash Point F does not flash Flash Point C does not flash -
Anti-Superoxide Dismutase (MnSOD) (DD-17)Cy3 antibody produced in rabbit
Кат. номер S1450-25UL S1450-200UL General descriptionThe SOD2 (superoxide dismutase 2) gene is mapped to human chromosome 6q25.3. The encoded protein functions as a tetramer and is localized to mitochondria.Immunogensynthetic peptide corresponding to amino acid residues 183-199 of human superoxide dismutase (MnSOD), conjugated to KLH. The corresponding sequence is conserved in many eukaryotes.ApplicationAnti-Superoxide Dismutase (MnSOD) (DD-17)-Cy3 antibody produced in rabbit has been used in immunohistochemistry.
Biochem/physiol Actions
Superoxide dismutase 2 (SOD2) is an antioxidant enzyme and catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen. The enzyme offers protection against oxidative damage induced by reactive oxygen species and is also radioprotective. The gene is associated with the metastasis and invasion process of salivary adenoid cystic carcinoma. Manganese SOD is involved in detoxification.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 200 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous solution species reactivity rat, human, mouse packaging antibody small pack of 25 µL concentration 1-2 mg/mL application(s) direct immunofluorescence: 2-5 µg/mL using human HeLa, rat NRK and mouse 3T3 cells. conjugate CY3 conjugate UniProt accession no. P04179, shipped in dry ice storage temp. 20°C Gene Information human ... SOD2(6648)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable -
Anti-Superoxide Dismutase (SOD) antibody, Mouse monoclonal
NACRES: NA.41
Кат. номер SAB4200807-25UL SAB4200807-100UL General descriptionSuperoxide Dismutase (SOD) is a family of metalloenzymes widely distributed in both plants and animals. Superoxide dismutases appear to protect cells against reactive free radicals by scavenging the superoxide radicals produced by ionization radiation or through other mechanisms. SOD enzyme catalyzes the conversion of single electron reduced species of molecular oxygen to hydrogen peroxide and oxygen. There are several classes of SOD that differ in their metal binding ability, distribution in different cell compartments, and sensitivity to various reagents. Among these, Cu, Zn superoxide dismutase (SOD1) is widely distributed and comprises 90% of the total SOD. This ubiquitous enzyme, which requires Cu and Zn for its activity, has great physiological significance and therapeutic potential.Immunogenrecombinant human copper-zinc superoxide dismutase (Cu-Zn-SOD).ApplicationMonoclonal Anti-Superoxide Dismutase (SOD) recognizes natural (human erythrocyte SOD), recombinant SOD (human Cu-Zn-SOD and human placental SOD) and the enzymatically inactive form of these enzymes. Reactivity has been observed with SOD from human, rat and dog origin, no reactivity was observed with SOD from bovine, bacillus stearothermophilus or E. coli (Fe or Mn) origin. The antibody is recommended to use in various immunological techniques, including ELISA, Immunohistochemistry and immunofluorescence.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.Other NotesThis product is for R&D use only, not for drug, household, or other uses.Параметрыbiological source mouse antibody form purified from hybridoma cell culture antibody product type primary antibodies clone SD-G6, monoclonal form buffered aqueous solution species reactivity rat, human, canine packaging antibody small pack of 25 µL application(s) indirect ELISA: 0.3-0.6 µg/mL using 5µg/ml Superoxide Dismutase from human erythrocytes for coating isotype IgG1 UniProt accession no. P00441, shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-SWAP-70
eCl@ss: 32160702
Кат. номер ABC1688-25UG ABC1688-100UG General descriptionSwitch-associated protein 70 (UniProt: Q9UH65; also known as SWAP-70) is encoded by the SWAP70 (also known as KIAA0640, HSPC321) gene (Gene ID: 23075) in human. SWAP-70 is a phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent guanine nucleotide exchange factor (GEF) that, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. SWAP-70 regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. It is expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG, but not IgM. It is also widely expressed in spleen, kidney, lung, and liver. SWAP-70 has an N-terminal putative EF-hand domain, a pleckstrin homology (PH) domain (aa 210-306) that binds phosphatidylinositol 3,4,5-trisphosphate and is responsible for membrane localization, a tri-partite coiled-coil region (aa 316-539), and a C-terminal F-actin-binding site through which it specifically binds non-muscle actin. (Ref.: Chacn-Martnez, CA et al. (2103). J. Biol. Chem. 288(40); 28687-28703).
Specificity
This rabbit polyclonal antibody detects human Switch-associated protein 70. It targets an epitope with in 12 amino acids from the N-terminal region.ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human Switch-associated protein 70.ApplicationImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected SWAP-70 in human tonsil and human lung tissue sections.
Anti-SWAP-70, Cat. No. ABC1688, is a rabbit polyclonal antibody that detects Switch-associated protein 70 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected SWAP-70 in Raji cell lysate.
Target description
~70 kDa observed; 69.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).Other NotesConcentration: Please refer to lot specific datasheet.Параметрыbiological source rabbit antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal purified by affinity chromatography species reactivity human species reactivity (predicted by homology) bovine (based on 100% sequence homology), mouse (based on 100% sequence homology) packaging antibody small pack of 25 µg application(s) immunohistochemistry: suitable (paraffin)","western blot: suitable NCBI accession no. NP_001284643, UniProt accession no. Q9UH65, Gene Information human ... SWAP70(23075)
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-Synemin (C-terminal region) antibody produced in rabbit
NACRES: NA.41
Кат. номер SAB4200138-25UL SAB4200138-200UL General descriptionSynemin is a 230kDa intermediate filament protein (IFp). It is expressed along with desmin and vimentin filaments in muscle cells. In mouse, synemin is encoded by the gene mapped in B5 region of chromosome 7. Alternative splicing of this gene produces three different isoforms with different molecular mass namely, high (180kDa), medium (150kDa) and low (41kDa). High and medium isoforms are expressed in embryonic mouse, whereas, low molecular weight protein is expressed in adult mouse. Synemin comprises 310-amino acid rod domain characteristic of IF proteins and 1,290 amino acid tail domain at its C- terminal.
Synemin exists in two isoforms, α and β.
Specificity
Anti-Synemin (C-terminal region) specifically recognizes rat synemin.ApplicationAnti-Synemin (C-terminal region) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
α-Synemin is essential for the stabilization of junctional complexes at sarcolemma, in neonates. β-Synemin controls the connection of desmin with Z-disks. Synemin is also involved in protein phosphorylation, for regulating key signaling cascades in the heart. Mild myopathy has been observed in synemin knockout mice.
Synemin in association with either desmin or vimentin plays a vital role in cytoskeletal organization and also facilitates function of intermediate filaments in erythrocytes and muscle. Interaction of synemin and vinculin aids in attachment of intermediate filaments (Ifs) to the cell membrane.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C.For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation.Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous solution mol wt ~170 kDa species reactivity rat packaging antibody small pack of 25 μL concentration ~1.5 mg/mL application(s) western blot: 1.5-3.0 μg/mL using using rat bladder extract (S1 fraction). conjugate unconjugated shipped in dry ice storage temp. −20°C Gene Information rat ... Synm(233335)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable -
Anti-T antibody produced in rabbit
Human Protein Atlas Number: HPA003322 Human Protein Atlas characterization data
Кат. номер HPA003322-100UL HPA003322-25UL ImmunogenBrachyury protein recombinant protein epitope signature tag (PrEST)ApplicationAll Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org),and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols, and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige,.
Biochem/physiol Actions
T gene encodes a protein called Brachyury protein that belongs to the T-box family of transcription factors. It plays an essential role in the formation and differentiation of the mesoderm and the axial development during embryogenesis of all vertebrates. It binds to a specific palindromic T-site in the DNA via its N-terminus region called the T-box and regulates transcription of certain genes. Polymorphism in the gene has been associated with a risk of spina bifida. It plays an important role in notochord development and a duplication of this gene confers major susceptibility to familial chordoma. Defects in the gene cause SAVA (Sacral agenesis with vertebral anomalies) characterized by sacral agenesis, abnormal ossification of the vertebral bodies and a persistent notochordal canal.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
LinkageCorresponding Antigen APREST84792,.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azideLegal InformationPrestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous glycerol solution species reactivity human packaging antibody small pack of 25 µL application(s) immunofluorescence: 0.25-2 µg/mL immunogen sequence QQPGYSQWGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQY conjugate unconjugated UniProt accession no. O15178, shipped in wet ice storage temp. 20°C Gene Information human ... T(6862)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-TAB-2 Antibody, clone 1D5.2
eCl@ss: 32160702
Кат. номер MABS1894-25UG MABS1894-100UG General descriptionTGF-beta-activated kinase 1 and MAP3K7-binding protein 2 (UniProt: Q9NYJ8; also known as Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, TAB-2, TGF-beta-activated kinase 1-binding protein 2) is encoded by the TAB2 (also known as KIAA0733, MAP3K7IP2) gene (Gene ID: 23118) in human. TAB-2 is a peripheral membrane protein that serves as an adapter protein that links MAP3K7/TAK1 and TRAF6. Two isoforms of TAB-2 have been reported that are produced by alternative splicing. TAB-2 promotes MAP3K7 activation in the IL-1 signaling pathway. Following IL-1 stimulation it translocates from the membrane to cytosol. TAB-2 is widely expressed. In the embryo, it is expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves. TAB-2 contains a RanBP2-type zinc finger domain (aa 663-693) that mediates binding to two consecutive Lysine 63-linked ubituitins. The binding of Lysine 63-linked polyubiquitin chains to TAB-2 promotes autophosphorylation of MAP3K7 at Threonine 187. Mutations in TAB2 gene are reported to cause multiple congenital heart defects characterized by congenital developmental abnormalities involving structures of the heart, including left ventricular outflow tract obstruction, sub-aortic stenosis, atrial fibrillation, bicuspid aortic valve and aortic dilation.
Specificity
Clone 1D5.2 specifically detects human TGF-beta-activated kinase 1 and MAP3K7-binding protein 2. It targets an epitope within 133 amino acids from the C-terminal region.ImmunogenGST/His-tagged recombinant fragment corresponding to 133 amino acids from the C-terminal region of human TAK1-binding protein 2 (TAB-2)ApplicationWestern Blotting Analysis: 1 µg/mL from a representative lot detected TAB-2 in 10 µg of Raji cell lysate.
Anti-TAB-2, clone 1D5.2, Cat. No. MABS1894, is a mouse monoclonal antibody that detects TAK1-binding protein 2 and has been tested for use in Western Blotting.
Quality
Evaluated by Western Blotting in TF-1 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TAB-2 in 10 µg of TF-1 cell lysate.
Target description
~77 kDa observed; 76.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: PurifiedOther NotesConcentration: Please refer to lot specific datasheet.Параметрыbiological source mouse antibody form purified antibody antibody product type primary antibodies clone 1D5.2, monoclonal species reactivity human packaging antibody small pack of 25 µg application(s) western blot: suitable isotype IgG2b NCBI accession no. NP_001278963, UniProt accession no. Q9NYJ8, Gene Information human ... TAB2(23118)
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-TAF-15 Antibody, clone 12F11
eCl@ss: 32160702
Кат. номер MABE1918-25UG MABE1918-100UG General descriptionTATA-binding protein-associated factor 2N (UniProt: Q92804; also known as 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56, TAF15) is encoded by the TAF15 (also known as RBP56, TAF2N) gene (Gene ID: 8148) in human. TAF15 is a ubiquitously distributed protein that is found in all fetal and adult tissues. It is a RNA and ssDNA-binding protein that plays specific roles during transcription initiation at distinct promoters and can enter the preinitiation complex together with the RNA polymerase II (Pol II). TAF15 shuttles between the nucleus and the cytoplasm. It can be dimethylated by protein arginine N-methyltransferase 1 (PRMT1) at Arg206 to asymmetric dimethylarginine. This methylation favors its nuclear localization and it also considered to be essential for its gene regulatory function. TAF15 contains an N-terminal activation domain, an RNA recognition motif (RRM) and many Arg-Gly-Gly (RGG) repeats at its C-terminal end. Its N-terminus serves as an essential transforming domain in the fusion oncoprotein created by chromosomal translocation in certain human chondrosarcomas. Depletion of TAF15 has a growth-inhibitory effect and results in increased apoptosis. (Ref.: Ballarino, M et al. (2013). Oncogene 32(39); 4646-4655; Jobert, L., et al. (2008). Exp. Cell res. 315(7);1273-1286).
Specificity
Clone 12F11 is a rat monoclonal antibody that detects human TATA-binding protein-associated factor 2N (TAF15). It targets an epitope with in 20 amino acids from the C-terminal half.ImmunogenOvalbumin-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human TATA-binding protein-associated factor 2N (TAF15).ApplicationAnti-TAF-15, clone 12F11, Cat. No. MABE1918, is a highly specific rat monoclonal antibody that targets TATA-binding protein-associated factor 2N and has been tested for use in ELISA and Western Blotting.
Western Blotting Analysis: A representative lot detected TAF-15 in Western Blotting applications (Suarez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).
ELISA Analysis: A representative lot detected TAF-15 in ELISA applications (Suarez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TAF-15 in HEK293 cell lysate.
Target description
~68 kDa observed; 61.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rat antibody form purified immunoglobulin antibody product type primary antibodies clone 12F11, monoclonal species reactivity human packaging antibody small pack of 25 µg application(s) ELISA: suitable","western blot: suitable isotype IgG2c NCBI accession no. NP_631961.1, UniProt accession no. Q92804, Gene Information human ... TAF15(8148)
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-TAPA-1 (CD81) Antibody, clone 5A6
eCl@ss: 32160702 NACRES: NA.41
Кат. номер MABF2061 MABF2061-25UL General descriptionCD81 antigen (UniProt: P60033; also known as 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD81) is encoded by the CD81 (also known as TAPA1, TSPAN28) gene (Gene ID: 975) in human. TAPA-1 is a nonglycosylated, homodimeric, multi-pass membrane protein of the tetraspanin (TM4SF) family. It is expressed in a wide range of cells, including hematolymphoid, neuroectodermal, and mesenchymal tumor cell lines and is reported to play a role in the regulation of lymphoma cell growth. It contains four transmembrane domains, two extracellular loops, and short amino and carboxyl termini that are intracellular. TAPA-1 is shown to affect cell adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells it is a part of a complex involving CD21, CD19, and Leu13 and this complex is reported to reduce the threshold for B cell activation via the B cell receptor by bridging antigen specific recognition and CD21-mediated complement recognition. On T cells, it is shown to associate with CD4 and CD8 and provides a costimulatory signal with CD3. It is also reported to act as a receptor for hepatitis C virus (HCV) in hepatocytes. Mutations in CD81 gene cause common variable immunodeficiency 6 (CVID6) that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. (Levy S., et al. (1998). Annu. Rev. Immunol.16; 89-109; Oren, R., et al. (1990). Mol. Cell Biol. 10(8); 4007-15).
Specificity
Clone 5A6 detects TAPA-1 (CD81) in human cells.ImmunogenOCI-LY8 whole cells (Cellosaurus cell line).ApplicationAnti-TAPA-1 (CD81), clone 5A6, Cat. No. MABF2061, is a mouse monoclonal antibody that detects TAPA-1 (CD81) and has been tested for use in Flow Cytometry, Inhibition assay, Immunoprecipitation and Western Blotting.
Western Blotting Analysis: A representative lot detected TAPA-1 (CD81) in Western Blotting applications (Clark, K.L., et. al. (2004). J Biol Chem. 279(19):19401-6).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TAPA-1 (CD81) in Immunoprecipitation applications (Clark, K.L., et. al. (2004). J Biol Chem. 279(19):19401-6; Oren, R., et. al. (1990). Mol Cell Biol. 10(8):4007-15).
Flow Cytometry Analysis: A representative lot detected TAPA-1 (CD81) in Flow Cytometry applications (van Zelm, M.C., et. al. (2010). J Clin Invest. 120(4):1265-74; Oren, R., et. al. (1990). Mol Cell Biol. 10(8):4007-15).
Inhibition: A representative lot inhibited the growth and proliferation of OCI-LY8 cell line. (Oren, R., et. al. (1990). Mol Cell Biol. 10(8):4007-15).
Research Category
Inflammation & Immunology
Quality
Evaluated by Flow Cytometry in HUH-7 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected TAPA-1 (CD81) in one million HUH-7 cells.
Target description
25.81 kDa calculated.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 containing PBS without azide.
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 5A6, monoclonal species reactivity human packaging antibody small pack of 25 µL application(s) flow cytometry: suitable","immunoprecipitation (IP): suitable","inhibition assay: suitable","western blot: suitable isotype IgG1 NCBI accession no. NP_001284578, UniProt accession no. P60033, shipped in ambient Gene Information human ... CD81(975)
Safety InformationFlash Point F does not flash Flash Point C does not flash -
Anti-Tapasin
eCl@ss: 32160702 NACRES: NA.41
Кат. номер ABF1067 ABF1067-25UL General descriptionTapasin (UniProt: Q9R233; also known as TPN, TPSN, TAP-associated protein, TAP-binding protein) is encoded by the Tapbp (also known as Tapa) gene (Gene ID: 21356) in the murine species. Tapasin is a single-pass type I membrane glycoprotein protein that is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide. It is synthesized with a signal peptide of 23 amino acids, which is subsequently cleaved to generate mature Tapasin. It assists in loading of antigenic peptides on to class I molecules in the endoplasmic reticulum (ER). It is also reported to support the binding of high-affinity peptides to MHC class I in conjunction with ERp57. Defects in Tapasin expression are linked to destabilization of the MHC class I loading complex and diminution in the expression of MHC molecules at the cell surface. Tapasin is reportedly down-regulated in several human carcinomas and its re-expression in Tapasin-deficient cancer cells restores the expression of MHC class I antigen complexes and augment cancer cell immunogenicity, which helps in longer survival in animals bearing metastatic tumors. (Ref.: Lou, Y et al. (2008). Clin Cancer Res. 14(5); 1494-1501).
Specificity
This rabbit polyclonal antibody detects Tapasin in murine cells. It targets an epitope within 20 amino acids from the C-terminal region.ImmunogenEpitope: C-terminus
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine Tapasin.ApplicationAnti-Tapasin Antibody, Cat. No. ABF1067, is a rabbit polyclonal antibody that detects Tapasin in murine cells and has been tested for use in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Tapasin in K42 cells (Lum, R., et. al. (2016). J Biol Chem. 291(37):19631-41) and L-cells and BW5147 thymoma cells (Suh, W.K., et. al. (1999). J Immunol. 162(3):1530-40).
Western Blotting Analysis: A representative lot detected Tapasin in CMT.64 cells treated with IFN- (Lou, Y., et. al. (2008). Clin Cancer Res. 14(5):1494-501).
Research Category
Inflammation & Immunology
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tapasin in 10 µg of NIH/3T3 cell lysate.
Target description
~51 kDa observed; 49.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form unpurified antibody product type primary antibodies clone polyclonal species reactivity mouse packaging antibody small pack of 25 µL application(s) immunoprecipitation (IP): suitable","western blot: suitable isotype IgG NCBI accession no. NP_033344, UniProt accession no. Q9R233,
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-TARBP2 antibody produced in rabbit
MDL number: MFCD08705573 NACRES: NA.41
Кат. номер SAB4200111-25UL SAB4200111-200UL General descriptionThe transactivating response (TAR) RNA-binding protein (TRBP) (also known as TARBP2) gene is mapped to human chromosome 12q13.13. The encoded protein exists in two isoforms, TRBP1 and TRBP2, where TRBP2 has 21 additional amino acids. It is composed of two double stranded RNA binding domains (dsRBDs), a KR-helix motif within dsRBD2 and a Medipal domain that binds Moesin-ezrin-radixin-like protein (Merlin), Dicer and protein activator of the interferon-induced protein kinase (PACT). TRBP is a component of the RNA-induced silencing complex (RISC) loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), composed of Dicer and argonaute (Ago).
Specificity
Anti-TRBP2 recognizes human TRBP2.Immunogensynthetic peptide corresponding to amino acids 183-195 of human TRBP2 conjugated to KLH. The corresponding sequence is identical in mouse and rat.ApplicationAnti-TARBP2 has been used in immunoblotting and might also be used in immunofluorescence.
Biochem/physiol Actions
The transactivating response (TAR) RNA-binding protein (TRBP) (also known as TARBP2) binds and activates the human immunodeficiency virus (HIV)-1 with TAR RNA. It plays a role in spermatogenesis, cell growth, oncogenesis and viral replication. Targeted disruption of the TARBP2 gene, causes growth defects, sterility problems and severe oligospermic effects in mice.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыantibody form affinity isolated antibody Quality Level 200 antibody product type primary antibodies clone polyclonal form buffered aqueous solution mol wt antigen ~30 kDa species reactivity human packaging antibody small pack of 25 µL concentration ~1.0 mg/mL application(s) indirect immunofluorescence: 2.5-5 µg/mL using paraformaldehyde fixed HeLa cells","western blot: 2-4 µg/mL using lysates of HEK-293T cells conjugate unconjugated UniProt accession no. Q15633, shipped in dry ice storage temp. 20°C Gene Information human ... TARBP2(6895)
mouse ... 21357(21357)
rat ... Tarbp2(363006)
Safety InformationRIDADR NONH for all modes of transport -
Anti-TARDBP antibody produced in rabbit
Human Protein Atlas Number: HPA017284 Human Protein Atlas characterization data
Кат. номер HPA017284-100UL HPA017284-25UL General descriptionTARDBP (TAR DNA binding protein) is a nuclear localized, heterogeneous, highly conserved ribonucleo protein. It is widely expressed in tissues, including heart, lung, liver, spleen, kidney, muscle, and brain. It consists of two RNA-recognition motifs and a glycine-rich C-terminal sequence.ImmunogenTAR DNA-binding protein 43 recombinant protein epitope signature tag (PrEST)ApplicationAnti-TARDBP antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org),. Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols, and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige,.
Biochem/physiol Actions
TARDBP (TAR DNA binding protein) functions as a transcriptional gene and is involved in various processes such as RNA splicing of the cystic fibrosis transmembrane conductance regulator gene. It has been reported that TARDBP may play an important role in neurodegeneration. Abnormal aggregation of TARDBP causes an adult-onset neurological disorder, amyotrophic lateral sclerosis (ALS), that leads to the degeneration of motor neurons.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
LinkageCorresponding Antigen APREST71040,.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azideLegal InformationPrestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous glycerol solution species reactivity human packaging antibody small pack of 25 µL application(s) immunoblotting: 0.04-0.4 µg/mL","immunofluorescence: 0.25-2 µg/mL","immunohistochemistry: 1:200-1:500 immunogen sequence VTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNN conjugate unconjugated UniProt accession no. Q13148, shipped in wet ice storage temp. 20°C Gene Information human ... TARDBP(23435)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 -
Anti-Tau (TauC4 Antibody, 354-369)
eCl@ss: 32160702
Кат. номер ABN2178-25UL ABN2178-100UL General descriptionMicrotubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Microtubule-associated protein tau is shown to be expressed in neurons. It is mostly found in axons, in the cytosol, and in association with plasma membrane components. Nine isoforms of Tau have been described that are generated by alternative splicing. Tau proteins promote microtubule assembly and stability and may also be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, thus serving as a linker protein between both. The short isoforms are reported to allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease (AD), the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau. In AD brain Tau accumulates in both the somatodendritic and axonal domains of neurons and it also accumulates in the soma as neurofibrillary tangles (NFTs). Tau oligomers are reported to be neurotoxic when applied extracellularly to cultured neuronal cells and can induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. It has been reported that most of the C-terminus of Tau (aa 243-406) in PHFs can be cleaved by trypsin, however the TauC4 region is highly resistant to trypsin action. (Ref.: Taniguchi Watanabe, S., et al. (2016). Acta Neuropathol. 131(2); 267-280; Ando K., et al. (2010). Biochem. Soc. Trans. 38(4); 1001-1005).
Specificity
This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Epitope: unknownApplicationResearch Category
Neuroscience
Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).
Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Anti-Tau (TauC4, 354-369), Cat. No. ABN2178, is a highly specific rabbit polyclonal antibody that targets TauC4 region of Tau and has been tested for use in Electron Microscopy and Western Blotting.
Quality
Evaluated by Western Blotting in Recombinant full-length 4-repeat (4R) tau (human 4R1N).
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TauC4 region in full length recombinant human tau (human 4R1N).
Target description
~55 kDa observed; 45.85 kDa calculated.
Physical form
Rabbit polyclonal antiserum with 0.1% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit antibody form serum antibody product type primary antibodies clone polyclonal species reactivity human species reactivity (predicted by homology) rat (based on 100% sequence homology), mouse (based on 100% sequence homology) packaging antibody small pack of 25 µL application(s) electron microscopy: suitable","western blot: suitable isotype IgG NCBI accession no. NP_005901, UniProt accession no. P10636-8, -
Anti-Tau Antibody, clone 2A1-2E1
eCl@ss: 32160702
Кат. номер MABN2472-25UG MABN2472-100UG General descriptionMicrotubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Microtubule-associated protein tau is shown to be expressed in neurons. It is mostly found in axons, in the cytosol, and in association with plasma membrane components. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. Nine isoforms of Tau have been described that are generated by alternative splicing. Tau proteins promote microtubule assembly and stability, and may also be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, thus serving as a linker protein between both. The short isoforms are reported to allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease (AD), the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau. In AD brain Tau accumulates in both the somatodendritic and axonal domains of neurons and it also accumulates in the soma as neurofibrillary tangles (NFTs). Tau oligomers are reported to be neurotoxic when applied extracellularly to cultured neuronal cells and can induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo.
Specificity
Clone 2A1-2E1 detects Microtubule-associated protein tau in human and murine brain. It targets an epitope within 19 amino acids from the N-termnal half.ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Microtubule-associated protein tau.ApplicationAnti-Tau, clone 2A1-2E1, Cat. No. MABN2472, is a highly specific mouse monoclonal antibody that targets Microtubule-associated protein tau and has been tested for use in Immunohistochemistry and Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A representative lot detected Tau in Western Blotting applications (Ishigaki, S., et. al. (2017). Cell Rep. 18(5):1118-1131).
Immunohistochemistry Analysis: A representative lot detected Tau in Immunohistochemistry applications (Ishigaki, S., et. al. (2017). Cell Rep. 18(5):1118-1131).
Quality
Evaluated by Western Blotting in human Tau-441 recombinant protein lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected Tau in human Tau-441 recombinant protein lysates.
Target description
~70 kDa observed; 78.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone 2A1-2E1, monoclonal species reactivity mouse, human packaging antibody small pack of 25 µg application(s) immunohistochemistry: suitable","western blot: suitable isotype IgG2a NCBI accession no. NP_058519, UniProt accession no. P10636,
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable -
Anti-Tau antibody, Mouse monoclonal
MDL number: MFCD07370943 NACRES: NA.41
Кат. номер T9450-25UL T9450-100UL T9450-200UL General descriptionTau is a family of microtubule-associated proteins thought to regulate the stability and organization of microtubules in neuronal cells. The tau protein family is derived from alternative mRNA splice variants that originate from a single gene, and result in mature proteins that vary in size from 352 to 441 amino acids (45 to 60 kDa). Tau loses microtubule-binding activity and aggregates into paired helical filaments (PHFs) in neurodegenerative disorders. PHFs are the basic structural components of neurofibrillary tangles (NFTs). NFT accumulation correlates with the clinical progression of Alzheimer′s disease. Phosphorylation can affect the functional properties of tau and hyperphosphorylation of tau may result in the loss of taus microtubule binding activity and the formation of the insoluble aggregates. Hyperphosphorylation and nonenzymatic glycosylation are posttranslational modifications detected in PHF-tau, and numerous sites of hyperphosphorylation of both normal and PHF-tau have been identified.
Tau (τ), also known as MAPT (microtubule associated protein tau), is encoded by the gene mapped to human chromosome 17q21.3. It is highly expressed in neurons, but is most prominent in axons.
Specificity
Mouse monoclonal clone Tau46 anti-Tau antibody recognizes bovine, rat, human, and mouse Tau (approx. 45 to 60 kDa). The antibody recognizes a phosphorylation-independent epitope in amino acids 404-441 (human). The antibody recognizes all six isoforms of Tau and may cross react with MAP2 protein.Immunogenbovine Tau.ApplicationAnti-Tau antibody, Mouse monoclonal has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
Mouse monoclonal clone Tau46 anti-Tau antibody is useful in ELISA, immunoblotting, immunogold labeling, immunopreciptation as well as immunohistochemistry. It is used as a probe to determine the presence and roles of Tau protein in the regulation of the stability and organization of microtubules in neuronal cells.
Biochem/physiol Actions
Tau (τ) plays an essential role in the assembly and maintenance of microtubule structure. Deletion of tau (τ) results in developmental delay and learning disability. The gene expression is associated with the development of Alzheimer′s disease (AD). Genetic variation in τ gene increases the risk of susceptibility to the sporadic tauopathies, progressive supranuclear palsy (PSP) and corticobasal degeneration.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone Tau46, monoclonal form buffered aqueous solution species reactivity bovine, rat, human, mouse packaging antibody small pack of 25 µL concentration ~2 mg/mL application(s) immunohistochemistry: suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.5-1 µg/mL using mouse brain extract isotype IgG1 conjugate unconjugated UniProt accession no. P10636, shipped in dry ice storage temp. 20°C Gene Information human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable -
Anti-Tau-1 Antibody, clone PC1C6
eCl@ss: 32160702 NACRES: NA.41
Кат. номер MAB3420 MAB3420-25UG MAB3420-100UG General descriptionTau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)
Specificity
Binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE). However there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202 {Szendrei, et al 1993; http://www.ncbi.nlm.nih.gov/entrez/query.fcgi-cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7680727}. Also see Billingsley & Kincaid, 1997 Biochem J 323:577-591 for additional mapping information on PC1C6.ImmunogenPurified denatured bovine microtubule associated proteins.ApplicationWestern blot: Bovine brain microtubule proteins purified by two cycles of assembly and disassembly (9) are dissolved in SDS-PAGE sample buffer. Five micrograms of the microtuble preparation per lane is loaded onto a 4% to 20% SDS-PAGE gradient gel along side molecular weight markers (14.3 - 200 kD). After separation by electrophoresis, the proteins are blotted onto nitrocellulose. Tau is detected as a series of 5 bands (52-68 kD) with approximately 5 ng/mL of anti-tau1.
Immunofluorescence: A 1:1000 dilution of this antibody detected Tau in mouse primary neurons. (Basnet, N., et al. (2018). Nat. Cell Biol. 20(10); 1172-1180.
Immunohistochemistry: 5 µg/mL; stains axons in tissue primarily, however in culture Tau expression is not restricted to just axons.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Protocol
Dephosphorylation of tissue sections (optional)
Dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimers brain tissue with anti-tau-1 (6). This treatment changes the staining pattern of anti-tau-1 to include cell bodies, dendrites and axons of neurons. In untreated samples, anti-tau-1 stains axons only.
1. Incubate tissue sections at +32°C for 2.5 hours with constant agitation in the following solution: 100 mM Tris-HCl, pH 8.0; 130 units/mL alkaline phosphatase, 1 mM PMSF, 10 µg/mL pepstatin and 10 µg/mL leupeptin.
2. Rinse sections twice, 3 min per rinse, with 100 mM Tris-HCl, pH 8.0.
Anti-tau-1 staining
1. Block non-specific binding by incubating sections in PBS containing 1% (v/v) normal animal serum, and 0.03% (w/v) Triton X-100. The animal serum should be from the same species as the secondary antibody.
2. Rinse 3 times with PBS, 3 min per rinse.
3. Incubate sections with anti-tau-1, approximately 5 µg/mL, diluted in PBS containing 1% (v/v) normal animal serum.
4. Wash with PBS, changing the solution 3 times over a 3 min period.
5. Detect with a standard secondary antibody detection system (10-13).
Research Category
Neuroscience
Anti-Tau-1 Antibody, clone PC1C6 is an antibody against Tau-1 for use in IH & WB with more than 65 product citations.
Research Sub Category
Neurodegenerative Diseases
Target description
5 bands (52–68 kDa)LinkageReplaces: AB1512
Physical form
Format: Purified
0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide
Protein A purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Alzheimers brain tissue (dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimer’s brain tissue) or human T98G glioblastoma cellsOther NotesConcentration: Please refer to the Certificate of Analysis for the lot-specific concentration.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметры