Monoclonal Anti-USP7 antibody produced in rat

NACRES:

NA.41

Кат. номер

SAB4200042-25UL

SAB4200042-200UL

Monoclonal Anti-USP7 (rat IgG2a isotype) is derived from the hybridoma USP 3D8 produced by the fusion of mouse myeloma cells (P3X63 AG 8.653) and splenocytes from rat immunized with a peptide.
Ubiquitin specific peptidase 7 (USP7) is a kind of deubiquitylating (DUB) enzyme, which regulates tumor protein p53-mouse double minute 2 homolog (p53-mdm2) pathway. USP7 gene is located on human chromosome 16p13.2.
Specificity
Monoclonal Anti-USP7 recognizes human and rat USP7.
Monoclonal Anti-USP7 antibody produced in rat may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions
Overexpression of the USP7 gene results in p53-mediated apoptosis. Ablation of the USP7 gene expression results in p53 accumulation as opposed to the expected destabilization of p53. This effect has been traced to the ability of USP7 to stabilize mouse double minute 2 homolog (MDM2), a ubiquitin ligase that promotes the degradation of p53. Therefore, USP7 appears to play multiple roles in regulating the p53-MDM2 pathway and in maintaining steady-state levels of p53 in the cell. USP7 is considered for treating hematopoietic tumors due to its role in p53 regulation.
Ubiquitin specific peptidase 7 (USP7) promotes oncogenesis and recognizes specific inhibitors. USP7 is overexpressed in hepatocellular carcinoma (HCC) tissues, which contributes to tumor invasion and proliferation. USP7 is important for osteogenic differentiation of human adipose-derived stem cells (hASCs). It is used for hASC based stem cell therapy and bone tissue engineering. USP7 plays complex roles in DNA regulation, p53 stress response and endosomal protein recycling.
Target description
USP7 is ubiquitin specific peptidase 7 (herpes virus-associated). It is associated with induction of apoptosis, interspecies interaction between organisms, protein deubiquitination, regulation of protein stability, and ubiquitin-dependent protein cataboli
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	USP 3D8, monoclonal
form	buffered aqueous solution
mol wt	antigen ~130 kDa
species reactivity	rat, human
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: 2-4 µg/mL using HeLa cell extracts
isotype	IgG2a
conjugate	unconjugated
UniProt accession no.	Q93009,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... USP7(7874)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable