Monoclonal Anti-Tumor Necrosis Factor- antibody produced in hamster

MDL number:	MFCD01632994
NACRES:	NA.41

Кат. номер

T2824-25UL

T2824-200UL

Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.
Specificity
Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.
purified recombinant mouse TNF-α.
Monoclonal Anti-Tumor Necrosis Factor- antibody produced in hamster has been used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• neutralization

Packaging
200 μL in glass insert
Biochem/physiol Actions
TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.
Physical form
Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	hamster
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	TN3-19.12, monoclonal
form	buffered aqueous solution
species reactivity	rat, mouse
packaging	antibody small pack of 25 µL
concentration	~2 mg/mL
application(s)	immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","neutralization: suitable","western blot: 0.25-0.5 µg/mL using recombinant mouse TNF-
conjugate	unconjugated
UniProt accession no.	P16599,
shipped in	dry ice
storage temp.	20°C
Gene Information	mouse ... Tnf(21926) rat ... Tnf(24835)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable