Monoclonal Anti--Tubulin Isotype III antibody produced in mouse

MDL number:	MFCD08705576
NACRES:	NA.41

Кат. номер

T5076-200UL

T5076-25UL

Tubulin β 3 class III (TUBB3) encodes for the microtubule protein tubulin β3, which is majorly expressed in neurons. The gene is located on human chromosome 16q24.3.
Tubulin is the main constituent of microtubules made up of two similar polypeptides α and β, each with 50 kD (approx.) molecular weight. It has several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin isotype III antibody can be used in microarray and western blotting. It can also be used in immunoblotting. Mouse anti-β-tubulin isotype III antibody reacts specifically with an epitope present on the β-tubulin (isotype III) of bovine, rat, mouse, boar and human but not with tubulin present in chicken brain preparation or in a chicken fibroblasts cell line.
peptide corresponding to the carboxy-terminal sequence of human β-tubulin isotype III, conjugated to BSA.
Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse has been used in immunolabelling and flow cytometry.
Monoclonal anti-β-tubulin isotype III antibody (diluted 1:500) can be used as a primary antibody in immunocytochemical analysis of neurons. It can also be used for the localization of isotype III of β-tubulin by several immunochemical assays like dot blot, ELISA and immunohistochemistry.
Biochem/physiol Actions
Tubulin β 3 class III (TUBB3) regulates neurogenesis and is involved in axon guidance and maintenance. Mutations in this gene is associated with hypoplasia of commissural axon (CA), congenital fibrosis of the extraocular muscles type 3 (CFEOM3) and dysgyria.
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	SDL.3D10, monoclonal
form	buffered aqueous solution
species reactivity	human, bovine, mouse, rat, boar
packaging	antibody small pack of 25 µL
concentration	~2 mg/mL
application(s)	dot blot: suitable","immunocytochemistry: suitable","immunohistochemistry: suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.1-0.2 µg/mL using rat brain extract
isotype	IgG2b
conjugate	unconjugated
UniProt accession no.	Q13509,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TUBB3(10381) mouse ... Tubb3(22152) rat ... Tubb3(246118)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable