Monoclonal Anti--Tubulin III antibody produced in mouse

MDL number:	MFCD01323870
NACRES:	NA.41

Кат. номер

T8660-25UL

T8660-100UL

T8660-.2ML

T8660-.5ML

Monoclonal Anti-μ Tubulin Isotype III (mouse IgG2b isotype) is derived from the SDL.3D10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.
Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.
Specificity
The antibody specifically recognizes an epitope located on isotype III of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. The antibody can stimulate microtubule assembly when reconstituted with tubulin and either tau or MAP2.
synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA
It has also been used in immunofluorescence analysis.
Monoclonal Anti--Tubulin III antibody produced in mouse has been used in following studies:

• Immunocytochemistry.
• Flow cytometry.
• Immunoblotting.

Biochem/physiol Actions
Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	ascites fluid
antibody product type	primary antibodies
clone	SDL.3D10, monoclonal
contains	15 mM sodium azide
species reactivity	bovine, boar, rat, human
packaging	antibody small pack of 25 µL
enhanced validation	knockout
application(s)	indirect ELISA: suitable","western blot: 1:400 using a rat brain extract
isotype	IgG2b
conjugate	unconjugated
UniProt accession no.	Q13509,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TUBB3(10381) rat ... Tubb3(246118)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable