Monoclonal Anti--Tubulin antibody produced in mouse

MDL number:	MFCD00677366
NACRES:	NA.41

Кат. номер

T5326-200UL

T5326-100UL

T5326-25UL

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.
Specificity
The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.
synthetic γ-tubulin peptide, conjugated to KLH
Monoclonal Anti--Tubulin antibody produced in mouse has been used in following studies:

• Western blot analysis
• Immunohistochemistry
• Immunocytology

Biochem/physiol Actions
-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, -tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of -tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of -tubulin is observed in lung cancer. Tubulin 2 (TUBG2) plays a vital role in cell growth. Aberration in the -tubulin gene alters microtubule assembly. The expression levels of -tubulin can be considered as an important prognostic indicator for patients with astrocytomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody (purified from hybridoma cell culture)
antibody product type	primary antibodies
clone	GTU-88, monoclonal
form	buffered aqueous solution
mol wt	antigen 48 kDa
species reactivity	bovine, mouse, rat, chicken, Xenopus, human, hamster, canine
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	immunocytochemistry: 1-2 µg/mL using HeLa cells","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 1-2 µg/mL using total cell extract of chicken fibroblasts
isotype	IgG1
UniProt accession no.	P23258,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TUBG1(7283) mouse ... Tubg1(103733) rat ... Tubg1(252921)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable