Monoclonal Anti-TRF1 antibody produced in mouse

MDL number:	MFCD03792434
NACRES:	NA.41

Кат. номер

T1948-25UL

T1948-100UL

T1948-200UL

Monoclonal Anti-TRF1 (mouse IgG1 isotype) is derived from the TRF-78 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a human TRF1 protein produced in baculovirus. TRF1 and TRF2 (TTAGGG repeat binding factors) are two major proteins that bind to human telomers. TRF1 has a DNA binding domain with high homology to the Myb family of transcription factors. Unlike the Myb family that contains only one DNA binding motif, TRF1 has multiple of this motif.
Specificity
Monoclonal Anti-TRF1 recognizes human TRF1.
human TRF1 protein produced in baculovirus.
Monoclonal Anti-TRF1 antibody produced in mouse has been used in Western blotting and enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
TRF1 (TTAGGG repeat binding factor 1) has a negative effect on the length of the telomer. Overexpression of TRF1 in cancer cells that contain telomerase activity, causes the shortening of the length of their telomers. While inhibition of TRF1 causes the elongation of telomers. It was shown that the level of TRF1 in the cells does not affect the expression of the telomerase protein. This suggests that TRF1 may act directly on the activity of the telomerase protein. Tankyrase is a protein that interacts with TRF1 and its C-terminal region is homologous to poly (adenosine dinucleotide phosphate (ADP)-ribose) polymerase (PARP). In response to DNA damage, the PARP protein mediates ADP-ribose polymers of protein acceptors. In vitro studies have shown that tankyrase is responsible for that polyribosylation of TRF1, which in turn abolishes its ability to bind telomers.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	TRF-78, monoclonal
form	buffered aqueous solution
mol wt	antigen ~70 kDa by SDS-PAGE
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~2 mg/mL
application(s)	indirect ELISA: suitable","microarray: suitable","western blot: 2-4 µg/mL using HeLa nuclear extract
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P54274,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TERF1(7013)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2