Monoclonal Anti-TIGAR antibody produced in mouse

NACRES:

NA.41

Кат. номер

T8828-25UL

T8828-200UL

TP53-induced glycolysis and apoptosis regulator (TIGAR) is a p53 target gene mapped to human chromosome 12p13-3. The gene contains six exons and it is characterized with two possible p53 binding sites, one upstream of the first exon (BS1) and one within the first intron (BS2).
Monoclonal Anti-TIGAR (mouse IgG2b isotype) is derived from the hybridoma 9C10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to a fragment in exon 6-encoding region of human TIGAR . TIGAR expression was found to correlate with fludarabine sensitivity in chronic lymphocytic leukemia (CLL) cells.
Monoclonal Anti-TIGAR antibody produced in mouse has been used in:

• enzyme linked immuno sorbent assay (ELISA)
• immunoblotting
• immunoprecipitation

Biochem/physiol Actions
TP53-induced glycolysis and apoptosis regulator (TIGAR) inhibits glycolysis by decreasing the fructose-2, 6-bisphosphate levels in cells. This p53 induced protein also plays a vital role in regulation of intracellular reactive oxygen species (ROS) levels. TIGAR expression is associated with oncogenesis and development of several human cancers. Overexpression of TIGAR is observed in colorectal cancer patients. Thus, TIGAR expression might function as a potential biomarker for diagnosis of colorectal cancer and it can act as a target for developing therapeutics for the treatment of colorectal cancer. Nullified expression of TIGAR, increases the radiosensitivity of tumor cells and also enhances the autophagy activity in cells with nutrient starvation or metabolic stress. TIGAR increases cancer cell survival rate in response to tumor chemotherapy by inhibiting both apoptosis and autophagy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	9C10, monoclonal
form	buffered aqueous solution
mol wt	antigen ~30 kDa
species reactivity	monkey, human
packaging	antibody small pack of 25 μL
concentration	~1.0 mg/mL
application(s)	immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using HeLa nuclear extract
isotype	IgG2b
conjugate	unconjugated
shipped in	dry ice
storage temp.	−20°C
Gene Information	human ... C12orf5(57103)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable