Monoclonal Anti-RNF4 antibody produced in mouse

Кат. номер

SAB4200645-25UL

SAB4200645-100UL

RNF4 (ring finger protein 4), initially identified as a transcriptional cofactor, is an E3 ubiquitin ligase. As this protein contains both the SUMO-interacting motif (SIM) and RING finger domains, it links sumoylation to ubiquitination.
synthetic peptide corresponding to a sequence at the C terminal region of human RNF4
Biochem/physiol Actions
RNF4 (ring finger protein 4) plays a wide variety of physiological roles, such as targeting of proteins for degradation, including promyelocytic leukemia protein (PML), CENP-I (centromere protein I), hypoxia-inducible factor (HIF), PARP-1 (poly [ADP-ribose] polymerase 1) etc. It also induces the base excision repair machinery, thus controlling DNA methylation. It is involved in DNA demethylation therefore, promoting gene transcription. RNF4 acts upon the oncogenic protein, Tax, of human T-cell leukemia virus type 1 (HTLV-1), and it regulates its nucleo-cytoplasmic localization. Arsenic trioxide (ATO) facilitates the sumoylation of promyelocytic leukemia (PML) bodies, thus, increasing their RNF4-mediated SUMO-dependent ubiquitylation and degradation. RNF4 functions as a co-regulator of androgen receptor-dependent transcription and is expressed in mice testis. Therefore, it might be involved in spermatid maturation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	RN.4, monoclonal
form	buffered aqueous solution
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	flow cytometry: 1-3 µg/test using HeLa cells","immunoblotting: 2-4 µg/mL using whole extracts of HEK-293 cells.","immunofluorescence: 0.25-0.5 µg/mL using HeLa cells.
isotype	IgG1
UniProt accession no.	P78317,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... RNF4(6047)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable