Monoclonal Anti-Rab7 antibody produced in mouse

MDL number:	MFCD08705596
NACRES:	NA.41

Кат. номер

R8779-100UL

R8779-200UL

R8779-25UL

Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). This family belongs to the rat sarcoma (Ras) superfamily of small GTPases. Rab proteins contain conserved regions involved in guanine nucleotide binding and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. The gene Rab7a in human chromosome is mapped to 3q21.3.
Specificity
Monoclonal Anti-Rab7 recognizes human, monkey, bovine, rat, mouse, and chicken Rab7.
Monoclonal Anti-Rab7 antibody produced in mouse may be used in ELISA, immunoblotting (~24 kDa), immunoprecipitation, immunohistochemistry, immunofluorescence and immunocytochemistry.
Biochem/physiol Actions
Rab GTPases are central regulators of membrane trafficking between different subcellular compartments in eukaryotic cells. The regulatory capacity of Rab GTPases depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Activation of Rab proteins is coupled to their association with intracellular membranes, allowing them to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin and tubulin-dependent vesicle movement, and membrane fusion. Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are Rab-interacting lysosome protein (RILP) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rab-7-interacting ring finger protein (Rabring7) and the hVPS34/p150 complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	Rab7-117, monoclonal
form	buffered aqueous solution
mol wt	~24 kDa
species reactivity	human, chicken, rat, mouse, bovine, monkey
packaging	antibody small pack of 25 µL
concentration	~2 mg/mL
application(s)	immunocytochemistry: suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.5-1 µg/mL using HeLa total cell extract
isotype	IgG2b
conjugate	unconjugated
UniProt accession no.	P51149,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... RAB7A(7879) mouse ... Rab7(19349) rat ... Rab7a(29448)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable