Monoclonal Anti-Notch3 antibody produced in rat

NACRES:

NA.41

Кат. номер

SAB4200125-25UL

SAB4200125-200UL

Monoclonal Anti-Notch3 (rat IgG2a isotype) is derived from the hybridoma NIZ 8G5 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from the immunized rat.
Members of the Notch gene family encode transmembrane receptors. Mammals have four Notch receptors, Notch1 through Notch4. Notch1 and Notch2 have the highest homology with each other, whereas Notch3 and Notch4 are structurally divergent, lacking the transactivation domain that is found in Notch1 and Notch2. The Notch3 receptor possesses 34 extracellular epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN12 repeats (LIN). It also contains two protein-protein interaction domains, the RAM domain (R), two nuclear localization signals (NLS), six ankyrin (ANK) repeats and a PEST sequence (P) at its cytoplasmic portion. The NOTCH3 gene is mapped to human chromosome 19p13.12.
Specificity
Monoclonal Anti-Notch3 recognizes human and rat Notch3.
human recombinant full length Notch3.
Monoclonal Anti-Notch3 antibody produced in rat is suitable for immunoblotting.
Biochem/physiol Actions
The Notch receptors are critical for various cell fate decisions. The Notch signaling plays a key role in the normal development of many tissues and cell types. It has diverse effects on differentiation, survival and/or proliferation that are highly dependent on the signal strength and cellular context. Variations in the NOTCH3 gene leads to vascular degenerative disease known as cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). In addition, upregulation of the NOTCH3 gene indicates active and relapsing human T-cell acute lymphoblastic leukemia (T-ALL).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	monoclonal
form	buffered aqueous solution
mol wt	antigen ~270 kDa
species reactivity	rat, human
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	western blot: 2-4 µg/mL using using Jurkat cell extracts
isotype	IgG2a
conjugate	unconjugated
UniProt accession no.	Q9UM47,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... NOTCH3(4854) rat ... Notch3(56761)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable