Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse

MDL number:	MFCD00164708
NACRES:	NA.41

Кат. номер

N0142-25UL

N0142-1VL

N0142-100UL

N0142-.2ML

N0142-.5ML

Neurofilaments are composed of three subunits, namely NEFL (light), NEFM (medium) and NEFH (heavy). NEFH (neurofilament heavy) gene encodes the most important neuron-specific intermediate filament of cytoskeleton in myelinated axons. This gene is mapped to human chromosome 22q12.2.
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques.
C-terminal segment of enzymatically dephosphorylated pig neurofilament 200.
Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody has been used in immunohistochemistry and western blotting.
Biochem/physiol Actions
The heavy polypeptide neurofilament encoded by neurofilament heavy chain (NEFH) gene participates in the maintenance of cytoskeleton and axonal architecture in the proximal axonal region of spinal motoneurons. Mutations in the NEFH gene has a key role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). NEFH gene is also identified to generate autosomal dominant axonal Charcot-Marie-tooth disease (CMT2cc).
Physical form
Supplied as ascites fluid containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	ascites fluid
antibody product type	primary antibodies
clone	N52, monoclonal
contains	15 mM sodium azide
species reactivity	wide range
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): 1:400 using rat cerebellum, microarray: suitable, western blot: 1:1,000 using rat brain extract
isotype	IgG1
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... NEFH(4744) mouse ... Nefh(380684) rat ... Nefh(24587)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable