Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse

MDL number:	MFCD00162703
NACRES:	NA.41

Кат. номер

M8421-25UL

M8421-.2ML

M8421-100UL

M8421-.5ML

Myosin is a 480 kDa protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200 kDa each) and four light chains (15-26 kDa). Myosin 7 (MYH7) is mapped to human chromosome 14q11.1.
Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin, purified from myofibrils isolated from human skeletal muscle, was used as the immunogen.1-3. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2.
Specificity
Reacts with human adult skeletal muscle slow myosin. Does not react with the fast myosin heavy chain nor with embryonic or neonatal rat skeletal myosins, nor with cat fetal/embryonic skeletal myosin. Also reacts with human adult type I skeletal muscle fibers and with cardiac muscle cells. No reactivity detected with human or rat adult smooth muscle or with non-muscle tissues.
Monoclonal Anti-Myosin (Skeletal, Slow) recognizes an epitope located on the heavy meromyosin portion of human adult skeletal muscle slow myosin. Cross-reactivity has been observed with human, bovine, sheep, goat, porcine, dog, cat, rabbit, hamster, guinea pig, rat, mouse, and chicken. In rat, the product is highly specific for the slow myosin heavy chain of adult skeletal muscle. It does not react with the fast myosin heavy chain. It does not cross-react with embryonic or neonatal rat skeletal myosins, nor with cat fetal/embryonic skeletal myosin. Using immunohistochemical staining, the antibody reacts with human adult type I skeletal muscle fibers and with cardiac muscle cells. It also reacts faintly with adult human C-Fibers, co-expressing myosin heavy chain I and IIa in variable ratios. The product shows no reactivity with human or rat adult smooth muscle or with non-muscle tissues.
Human skeletal muscle myosin purified from myofibrils.
Monoclonal Anti-Myosin (Skeletal, Slow) may be used for the localization of slow (Type I) myosin.
Monoclonal Anti-Myosin (Skeletal, Slow) antibody has been used in the detection of Myosin 7 using:

• light microscopy
• immunofluorescence staining
• immunoblotting
• ELISA
• solid-phase RIA
• immunohistology (frozen, formalin-fixed, paraffin-embedded and methacarn-fixed paraffin-embedded tissue sections)
• immunoelectronmicroscopy

Detection of slow myosin in formalin-fixed, paraffin-embedded, tissues is greatly enhanced by proteolytic digestion of the preparation.
Biochem/physiol Actions
Myosin molecules consist of two major regions: tail (rod) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type: skeletal, cardiac, smooth and in non-muscle. Myosin isoforms exist in different types of skeletal muscle, depending on the physiological function of the muscle. Mammalian muscle fibers are classified primarily into slow (I), fast-red (IIa) and fast-white (IIb) major types. Changes in the speed of muscle contraction brought about by neural influences result from changes in the pattern of expression of myosin and other myofibrillar genes.
Transient expression of different myosin isoforms occurs during fetal growth and development. Mutations in myosin 7 is associated with laing distal myopathy (LDM). Myosin 7 gene mutations results in muscular dystrophy diseases like scapuloperoneal myopathy. Mutations leads to storage of myosin protein aggregates in muscle, leading to myosin storage myopathy. Mutations in MYH7 is also associated with hypertrophic cardiomyopathy and in heart malformation disease called ebstein anomaly.
Physical form
Supplied as ascites fluid with 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	ascites fluid
antibody product type	primary antibodies
clone	NOQ7.5.4D, monoclonal
contains	15 mM sodium azide
species reactivity	chicken, feline, bovine, guinea pig, canine, human, pig, goat, rabbit, sheep, mouse, rat, hamster
packaging	antibody small pack of 25 µL
application(s)	electron microscopy: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using protease-digested, sections of rabbit tongue","indirect ELISA: suitable","radioimmunoassay: suitable","western blot: 1:5,000 using extract of rat or rabbit tongue
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P12883,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... MYH7(4625) mouse ... Myh7(140781) rat ... Myh7(29557)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable