Monoclonal Anti-MeCP2 antibody produced in mouse

MDL number:	MFCD02686372
NACRES:	NA.41

Кат. номер

M6818-200UL

M6818-100UL

M6818-25UL

Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).
synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.
Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunostaining
• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
• western blot (0.1-0.2 µg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper),
Western Blotting (1 paper),
Biochem/physiol Actions
Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	Mec-168, monoclonal
form	buffered aqueous solution
mol wt	antigen ~75 kDa
species reactivity	rat, mouse, human
packaging	antibody small pack of 25 µL
application(s)	immunocytochemistry: suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.1-0.2 µg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P51608,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... MECP2(4204) mouse ... Mecp2(17257) rat ... Mecp2(29386)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable