Monoclonal Anti--Amyloid antibody produced in mouse

MDL number:	MFCD00803867
NACRES:	NA.41

Кат. номер

A3981-100UL

A3981-200UL

A3981-25UL

The antibody reacts specifically with β-amyloid protein. The epitope recognized by the antibody resides within amino acids 1-12 of the β-amyloid protein. It specifically stains amyloid plaques within the cortex and amyloid deposits in blood vessels using formic acid-treated, formalin-fixed, paraffin-embedded, and Methacarn-fixed sections of human Alzheimer′s disease (AD) brain tissue.
β-amyloid gene is located on human chromosome 21q21.
The β-amyloid precursor protein (APP) is cleaved sequentially by the proteolytic enzymes β-and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42 and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides are degraded either via a re-uptake mechanism followed by endosomal degradation, or by an extracellular insulin degrading enzyme. Extracellular accumulation of Aβ leads to the formation of aggregates, fibrils and eventually amyloid deposits called neuritic plaques, which is the hallmark of Alzheimer′s disease (AD).
Synthetic β-amyloid peptide, conjugated to KLH.
The antibody is useful in immunohistochemistry, immunoblotting, ELISA, and competitive ELISA. Also, this antibody has been used to neutralize A assemblies in brains of transgenic mice expressing a mutant form of amyloid precursor protein, and for in vivo deep tissue imaging using near-IR optical spectrum.
Mouse monoclonal anti-ABETA was used to treat old WT PDAPP mice with amyloid accumulation and learning deficits in an attempt to improve learning and decrease accumulation, however no response was observed.
Monoclonal Anti-β-Amyloid antibody has been used in immunoprecipitation analysis, microarray-based immunoassay and microarray.
Biochem/physiol Actions
β-amyloid gene acts as the substrate of insulin-degrading enzyme (IDE). It plays a major role in the pathogenesis of Alzheimers disease (AD) and type 2 diabetes mellitus (DM2).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	BAM-10, monoclonal
form	buffered aqueous solution
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 µg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue","indirect ELISA: suitable","western blot: suitable
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P05067,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... APP(351), APP(351)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable