Monoclonal Anti-AGO2 antibody produced in rat

NACRES:

NA.41

Кат. номер

SAB4200085-200UL

SAB4200085-25UL

Monoclonal Anti-AGO2 (rat IgG2a isotype) is derived from the hybridoma 11A9 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to amino acids 1-15 of human AGO2. Monoclonal Anti-AGO2 recognizes human, monkey, bovine, rat and mouse AGO2. Argonaute 2 (Ago2) is encoded by the gene mapped to human chromosome 8q24.3. The encoded protein belongs to the Ago protein family. AGO2 is mainly localized on the diffuse cytoplasm and stress granules.
Monoclonal Anti-AGO2 antibody produced in rat has been used in:

• immunofluorescent localization
• western blot analysis
• immunoprecipitation.

Biochem/physiol Actions
Argonaute 2 (Ago2) plays a vital role in short interfering RNA-mediated gene silencing. The encoded protein is regulated at both the transcriptional and posttranslational level in human breast cancer cell lines. Ago2 and enhanced micro-RNA activity is involved in the tumorigenic progression of breast cancer cell lines. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC), head and neck squamous cell carcinoma patients.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Cell lysates should not be boiled before loading onto SDS-PAGE, but only warmed to 60°C for 15 min. In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	11A9, monoclonal
form	buffered aqueous solution
mol wt	antigen ~85 kDa
species reactivity	mouse, human, rat, monkey, bovine
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: 0.5-1.0 µg/mL using HeLa cell extracts
isotype	IgG2a
conjugate	unconjugated
UniProt accession no.	Q9UKV8,
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable