Monoclonal Anti-Actin (plant) antibody produced in mouse

MDL number:	MFCD00145889
NACRES:	NA.41

Кат. номер

A0480-200UL

A0480-25UL

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.
ACT8 protein.
Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:

• two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
• immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
• immunoblotting

Biochem/physiol Actions
In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	10-B3 (MAbGPa), monoclonal
mol wt	antigen ~45 kDa
species reactivity	plant
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 1-2 µg/mL using corn leaves extracts
isotype	IgG2b
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C
Gene Information	Arabidopsis thaliana ... ACT8(841347)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable