Monoclonal Anti-Acetylated Tubulin antibody produced in mouse

MDL number:	MFCD00164512
NACRES:	NA.41

Кат. номер

T7451-200UL

T7451-25UL

T7451-100UL

Monoclonal Anti-Acetylated Tubulin (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.
α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Specificity
The antibody has been used to detect acetylated -tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the 3 isoform of Chlamydomonas axonemal -tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of -tubulins, but the corre­sponding region is markedly divergent in some -tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.
acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).
Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in western blot and immunohistochemistry.
Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has also been used in quantitative dot blot, immunofluorescence, Western blot, immunocytochemistry, enzyme linked immunosorbent assay (ELISA), solid phase radioimmunoassay (RIA) and electron microscopy.
Biochem/physiol Actions
Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	6-11B-1, monoclonal
form	buffered aqueous solution
species reactivity	plant, hamster, rat, mouse, human, pig, monkey, frog, invertebrates, bovine, protista, chicken
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	dot blot: suitable","electron microscopy: suitable","immunohistochemistry: suitable","radioimmunoassay: suitable","western blot: 0.03-0.06 µg/mL using total rat brain extract
isotype	IgG2b
conjugate	unconjugated
Featured Industry	Research Pathology
UniProt accession no.	P68366,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TUBA4A(7277) mouse ... Tuba1a(22142) rat ... Tuba1a(64158)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable