Anti-XPD Antibody, clone 22TF2-2F6

eCl@ss:

32160702

Кат. номер

MABE1819-25UG

MABE1819-100UG

General transcription and DNA repair factor IIH helicase subunit XPD (UniProt: P18074; also known as EC: 3.6.4.12, TFIIH subunit XPD, Basic transcription factor 2 80 kDa subunit, BTF2 p80, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH 80 kDa subunit, TFIIH p80, Xeroderma pigmentosum group D-complementing protein) is encoded by the ERCC2 (also known as XPD, XPDC) gene (Gene ID: 2068) in human. XPD is an ATP-dependent 5-3 DNA helicase component of the general transcription and DNA repair factor IIH (TFIIH) core complex that is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA. Its ATP-binding region is localized to amino acids 7-283 and its nuclear localization is in amino acids 682-695. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH plays an essential role in transcription initiation. When the pre-initiation complex has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail of the largest subunit of RNA polymerase II by CDK-activating kinase (CAK) complex controls the initiation of transcription. XPD acts by forming a bridge between CAK and the core-TFIIH complex. Mutations in ERCC2 gene are known to cause Xeroderma pigmentosum complementation group D that is characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. The skin develops marked freckling and other pigmentation abnormalities.
Specificity
Clone 22TF2-2F6 detects General transcription and DNA repair factor IIH helicase subunit XPD (ERCC2) in human cells. It targets an epitope with in 32 amino acids from the C-terminal region.
Epitope: C-terminus
Ovalbumin-conjugated linear peptide corresponding to 32 amino acids from the C-terminal region of human General transcription and DNA repair factor IIH helicase subunit XPD (XPD/ERCC2).
Western Blotting Analysis: A representative lot detected XPD in Western Blotting applications (Alekseev, S., et. al. (2017). Mol Cell. 65(3):504-514.e4).
Immunocytochemistry Analysis: A representative lot detected XPD in Immunocytochmeistry applications (Vermeulen, W., et. al. (2000). Nat Genet. 26(3):307-13). .
Immunoprecipitation Analysis: A representative lot immunoprecipitated XPD in Immunoprecipitation applications (Coin, F., et. al. (1998). Nat Genet. 20(2):184-8; Seroz, T., et. al. (2000). Nucleic Acids Res. 28(22):4506-13).
Anti-XPD, clone 22TF2-2F6, Cat. No. MABE1819, is a highly specific mouse monoclonal antibody that targets human General transcription and DNA repair factor IIH helicase subunit XPD and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected XPD in HeLa cells.
Target description
86.91 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	22TF2-2F6, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000391.1,
UniProt accession no.	P18074,