Anti-von Willebrand Factor Antibody

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

AB7356-25UG

AB7356

von Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.
This Anti-von Willebrand Factor Antibody is validated for use in ELISA, Immunohistochemistry (IHC) for the detection of von Willebrand Factor.
Specificity
AB7356 is specific for vWF by ELISA against the human protein and by immunohistochemistry. The antibody is reactive in paraffin embedded tissues on human, rat, and mouse. Other species not tested. Antibody has not been evaluated by western blot analysis for reactivity to vWF.
Human von Willebrand Factor purified from plasma.
Research Category
Cell Structure
Immunohistochemistry Analysis: A representative lot detected von Willebrand Factor in Immunohistochemistry applications.
ELISA Analysis: A representative lot detected von Willebrand Factor in ELISA applications.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected von Willebrand Factor in human cerebral cortex, mouse hippocampus, rat cerebellum and human tonsil tissue sections.
Anti-von Willebrand Factor, Cat. No. AB7356, is a rabbit polyclonal antibody that detects von Willebrand Factor and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.
Research Sub Category
ECM Proteins
Quality
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Rabbit anti-vWF (AB7356) staining of Human Cerebral Cortex. Tissue pretreated with Citrate Buffer, ph 6.0, anti-vWF at 20 µg/mL, IHC-Select Detection with HRP-DAB.
Target description
250 kDa
Physical form
Format: Purified
Purified rabbit immunoglobulin in buffer containing 0.02 M PBS pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Protein A purified
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	polyclonal
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µg
Торговая марка	Chemicon®
application(s)	ELISA: suitable","immunohistochemistry: suitable (paraffin)
NCBI accession no.	NM_000552.3,
UniProt accession no.	P04275,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... F8(302470), VWF(7450)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable