Anti-Vimentin antibody, Mouse monoclonal

NACRES:

NA.41

Кат. номер

V6389-25UL

V6389-100UL

V6389-200UL

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the V9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with vimentin purified from pig eye lens.
Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α--helical amino- terminal “head” and carboxy-terminal “tail” domain.
Specificity
The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.
pig eye lens vimentin.
Monoclonal Anti-Vimentin antibody produced in mouse has been used in:

• immunofluorescence
• immunohistochemistry
• immunocytochemistry
• immunoblotting
• flow cytometry.

Monoclonal Anti-Vimentin antibody produced in mouse has been used in immunohistochemical analysis and western blot assay.
Biochem/physiol Actions
Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	V9, monoclonal
form	buffered aqueous solution
mol wt	antigen ~58 kDa
species reactivity	bovine, feline, chicken, dog, gerbil, rat, monkey, hamster, horse, pig, human
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	flow cytometry: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 µg/mL using human tissue","microarray: suitable","western blot: 0.2-0.5 µg/mL using human fibroblasts HS-68
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P08670,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... VIM(7431) rat ... Vim(81818)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable