- Производитель:
- Sigma-Aldrich
eCl@ss: | 32160702 |
NACRES: | NA.41 |
Кат. номер |
ABN1647 |
ABN1647-25UL |
Specificity
This rabbit polyclonal antibody detects Vesicular glutamate transporter 1 in Human, Mouse, and Rat brain. It targets an epitope with in 14 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGLUT1).
Immunohistochemistry Analysis: A representative lot detected VGLUT1 in prefrontal cortex of a representative control subject and AD patient (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).
Western Blotting Analysis: A representative lot detected VGLUT1 in glutamate transporter 1 in rat and human putamen extracts (Kashani, A., et. al. (2007). Neurobiol Agining 28(4):568-78) and prefrontal cortex of controls, MCI, early stage Alzheimer (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).
Immunofluorescence Analysis: A representative lot detected VGLUT1 in mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).
Immunofluorescence Analysis: A representative lot detected VGLUT1 in rat striatum (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Immunoautoradiography Analysis: A representative lot detected VGLUT1 in coronal sections of frontal rat brain (Herzog, E., et. al. (2004). Neuroscience. 123(4):983-1002) and rat brain sagittal sections (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Western Blotting Analysis: A representative lot detected VGLUT1 in BON cells stably expressing VGLUT1 (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Research Category
Neuroscience
Anti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VGLUT1 in 20 µg of mouse brain tissue lysate.
Target description
~55 kDa observed; 61.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide and 50% glycerol.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
Quality Level | 100 |
antibody form | unpurified |
antibody product type | primary antibodies |
clone | polyclonal |
species reactivity | rat, mouse, human |
species reactivity (predicted by homology) | bovine (based on 100% sequence homology) |
packaging | antibody small pack of 25 µL |
application(s) | immunoautoradiography: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","western blot: suitable |
isotype | IgG |
NCBI accession no. | NP_446311, |
UniProt accession no. | Q62634, |
shipped in | ambient |
WGK Germany | WGK 1 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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