Anti-VAC14 (C-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

SAB4200075-200UL

SAB4200075-25UL

VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.
Specificity
Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.
Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions
VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P2. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P2 pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.
VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.
Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~82 kDa
species reactivity	rat, mouse, human
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	immunoprecipitation (IP): 10-15 µg using A431 cell lysates","indirect immunofluorescence: 8-16 µg/mL using HeLa cells","western blot: 1-2 µg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction)
conjugate	unconjugated
UniProt accession no.	Q08AM6,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... VAC14(55697) rat ... Vac14(307842)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable