Anti-Utrophin A

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABN1739

ABN1739-25UG

Utrophin A (UniProt: E9Q6R7) is encoded by the Utrn gene in murine species. Utrophin is a large cytoskeletal protein, which is autosomally-encoded homologue of dystrophin, the protein product of the Duchenne muscular dystrophy (DMD) gene. It displays a sequence homology with dystrophin and possesses many of the protein-binding properties ascribed to dystrophin. In normal skeletal muscle, utrophin is located at the neuromuscular junction and dystrophin predominates at the sarcolemma. During development and in certain myopathies utrophin is also reported to be present at the sarcolemma and this redistribution is associated with higher levels of utrophin. Utrophin is found to co-localize with the acetylcholine receptors at the neuromuscular junctions and may participate in stabilizing the synaptic cytoskeleton. The utrophin mRNA contains two full-length species (named A- and B-utrophin), which are transcribed from different promoters. Hence, utrophin is a composite of A- and B-utrophin and only the A-utrophin is up-regulated in dystrophin-deficient striated muscle. This up-regulation is reported to occur post-transcriptionally. Utrophin A and B differ in their N-termini sequence. (Ref.: Blake, DJ et al. (1996). Brain Pathol. 6(1); 37-47; Weir, AP et al. (2002). J. Biol. Chem. 277 (47); 45285-90).
Specificity
This rabbit polyclonal antibody detects utrophin in human and mouse muscle tissue. It targets an epitope with in the first 26 amino acids from the N-terminal region.
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the first 26 amino acids from the N-terminal region of murine utrophin.
Anti-Utrophin A Antibody, Cat. No. ABN1739, is a highly specific rabbit polyclonal antibody that targets Utrophin and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Utrophin A in human skeletal muscle and human uterus tissue.
Immunofluorescence Analysis: A representative lot detected Utrophin A in quadriceps transverse sections in transgenic mice (Adams, M.E., et. al. (2010). J Neurosci. 30(33):11004-10).
Immunohistochemistry Analysis: A representative lot detected Utrophin A in frozen sections of the gastrocnemius muscles (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).
Western Blotting Analysis: representative lot detected Utrophin A in whole muscle lysates (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).
Western Blotting Analysis: A representative lot detected Utrophin A in muscle membrane extracts from mdx mice (Courtesy of Dr Marvin Adams at University of Washington).
Immunofluorescence Analysis: A representative lot detected Utrophin A in mouse neuromuscular junction (Courtesy of Dr Marvin Adams at University of Washington).
Research Category
Neuroscience
Quality
Evaluated by Immunohistochemistry in human prostate.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Utrophin A in human prostate tissue.
Target description
394.47 kDa calculated.
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	immunofluorescence: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_035812,
UniProt accession no.	E9Q6R7,
Gene Information	human ... UTRN(7402)

Flash Point F	does not flash
Flash Point C	does not flash