Anti-UPF3B

eCl@ss:

32160702

Кат. номер

ABE1900-25UG

ABE1900-100UG

Regulator of nonsense transcripts 3B (UniProt: Q9BZI7; also known as Nonsense mRNA reducing factor 3B, Up-frameshift suppressor 3 homolog B, UPF3B, hUpf3B, Up-frameshift suppressor 3 homolog on chromosome X, hUpf3p-X) is encoded by the UPF3B (also known as RENT3B, UPF3X) gene (Gene ID: 65109) in human. UPF3B is widely expressed, including in testis, uterus, heart, muscle, brain, spinal cord, and placenta. It shuttles between the nucleus and the cytoplasm. It is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serves as a link between the EJC core and NMD machinery. UPF3B recruits UPF2 at cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex is believed to activate NMD. In cooperation with UPF2 it stimulates both ATPase and RNA helicase activities of UPF1. Mutations in UPF3B gene have been linked to X-linked mental retardation that is characterized by significantly low general intellectual functioning associated with impairments in adaptive behavior, autistic features, poor musculature and other abnormalities. Two isoforms of UPF3B have been described that are produced by alternative splicing.
Specificity
This rabbit polyclonal antibody detects human Up-frameshift suppressor 3 homolog B (UPF3B). It targets an epitope with in 18 amino acids from the C-terminal half.
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human Up-frameshift suppressor 3 homolog B.
Epitope: unknown
Anti-UPF3B, Cat. No. ABE1900, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 3B (UPF3B) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Western Blotting Analysis: A representative lot detected UPF3B in lysate from HeLa cells overexpressing UPF3B. (Courtesy of Matthew Kim & Dr. Wilkinson Miles at University of California, San Diego).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected UPF3B in human testis and mouse testis tissue sections.
Western Blotting Analysis: 2 µg/mL from a representative lot detected UPF3B in lysate from HEK cells transfected with UPF3B vector, but not in HEK cells transfected with control vector.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in iPSCs and UPF3B-KO iPSCs.
Western Blotting Analysis: 2 µg/mL of this antibody detected UPF3B in lysate from iPSCs, but not in lysate from UPF3B knockout iPSCs.
Target description
~58 kDa observed; 57.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_542199,
UniProt accession no.	Q9BZI7,