Anti-UBE2A

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS2203

ABS2203-25UG

Ubiquitin-conjugating enzyme E2 A (UniProt: P49459; also known as EC 2.3.2.23; E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A) is encoded by the UBE2A (also known as RAD6A) gene (GeneID: 7319) in human. UBE2A belongs to the ubiquitin-conjugating enzyme family and accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1, it plays a role in transcription regulation by catalyzing the mono-ubiquitination of histone H2B at Lys-120 that provides a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, and telomeric silencing. It is also a prerequisite for H3K4me and H3K79me formation. Defects in UBE2A gene have been linked to mental retardation, X-linked, syndromic, Nascimento-type mental retardation that is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior.
Specificity
This rabbit polyclonal antibody detects Ubiquitin-conjugating enzyme E2 A (UBE2A) in HeLa cells and Mouse Kidney.
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Ubiquitin-conjugating enzyme E2 A (UBE2A).
Epitope: C-terminus
Anti-UBE2A Antibody, Cat. No. ABS2203, is a rabbit polyclonal antibody that detects UBE2A and has been tested for use Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A 1:500 dilution from a representative lot detected UBE2A in mouse kidney tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE2A in human pancreas, human cervical cancer, and human uterus tissues.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected UBE2A in HeLa cell lysate.
Target description
~17 kDa observed; 17.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	rat (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
NCBI accession no.	NP_003327,
UniProt accession no.	P49459,
shipped in	ambient
Gene Information	human ... UBE2A(7319)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable