Anti--Tubulin antibody, Mouse monoclonal

MDL number:	MFCD00145891
NACRES:	NA.41

Кат. номер

T8203-100UL

T8203-200UL

T8203-25UL

Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma AA13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with rat brain tubulin.
Tubulin 1a (TUBA1A) is a member of -tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development.
The encoded protein structure contains core of two -sheets enclosed by -helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.
rat brain tubulin.
Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.
The antibody has been used in various immunochemical techniques including immunoprecipitation, immunocytochemistry, and immunohistochemistry.
Biochem/physiol Actions
Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS).
Tubulin is the major building block of microtubules, which is a structural and mobile element in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Monoclonal antibodies recognizing α-tubulin, together with monoclonal antibodies to other tubulin types (α-tubulin isotypes I, II, and III, tyrosine tubulin and acetylated- α -tubulin) provide a specific and useful tool in studying the intracellular distribution of tubulin, and the static and dynamic aspects of the cytoskeleton. In addition, the various tubulin forms have been implicated in cancerous processes and are thus suggested as potential targets for cancer therapy.
Target description
Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	AA13, monoclonal
form	buffered aqueous solution
mol wt	antigen ~50 kDa
species reactivity	human, mouse, rat
packaging	antibody small pack of 25 µL
concentration	~2.0 mg/mL
application(s)	immunocytochemistry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","indirect ELISA: suitable","western blot: 1-2 µg/mL using Neuro-2a cell extract
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	Q71U36,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TUBA1A(7846) mouse ... Tuba1a(22142) rat ... Tuba1a(64158)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable