Anti-Tspan-33 Antibody, clone 29A6.2 Antibody, FITC conjugate

eCl@ss:

32160702

Кат. номер

MABT911F-25UL

MABT911F-100UL

Tetraspanin-33 (UniProt: Q8R3S2; also known as Tspan-33, Penumbra, mPen, Proerythroblast new membrane) is encoded by the Tspan33 (also known as Pen) gene (Gene ID: 232670) in murine species. Tetraspanins are evolutionarily conserved proteins and thus far 33 of them have been described in human and murine species. Tspan-33 is a multi-pass, disulfide-linked homodimeric membrane protein of the tetraspanin (TM4SF) family. It has been reported that Tspan-33 is highly expressed in activated B cells. Its expression has also been reported in malignant B cells. Two isoforms of Tspan-33 have been described that are produced by alternative splicing. Tspan-33 plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitors. Tspan-33 contains four transmembrane domains (aa 25-45, 65-85, 97-117, 236-256), three cytoplasmic domains (aa 1-24, 86-96, 257-283), and two extracellular domains (aa 46-64, 118-235). Several tetraspanins interact with specific membrane proteins via their second extracellular domain (aa 118-235) and incorporate them into tetraspanin-enriched microdomains to modulate the effectiveness of signal transduction by those membrane proteins. (Ref.: Luu, VP., et al. (2013). Clin. Immunol. 149(3): 388-399).
Specificity
Clone 29A6.2 specifically detects Tspan-33 and targets an epitope with in the extracellular domain.
Epitope: extracellular domain
BaF3 cells expressing full-length murine Tspan-33 fused with a Myc-His tag at the C-terminus.
Research Category
Cell Structure
Anti-Tspan-33, clone 29A6.2, FITC conjugate, Cat. No. MABT911F, is a mouse monoclonal antibody that detects Tetraspanin-33 and has been tested for use in Flow Cytometry.
Quality
Evaluated by Flow Cytometry in mouse splenocytes.
Flow Cytometry Analysis: 0.5 µg of this antibody detected Tspan-33 in 500,000 cells per mL mouse splenocytes.
Target description
31.55 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgM in PBS with 0.1% sodium azide, with 1.5% BSA.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	29A6.2, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgM
conjugate	FITC conjugate
NCBI accession no.	NP_666285.1,
UniProt accession no.	Q8R3S2,