Anti-Tropomyosin-1 Antibody, clone 7F8B8

eCl@ss:

32160702

Кат. номер

MABT1365-25UL

MABT1365-100UL

Tropomyosin alpha-1 chain (UniProt: P09493; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the TPM1 (also known as C15orf13, TMSA) gene (Gene ID: 7168) in human. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Ten different isoforms of Tropomyosin-1 have been described that are produced by alternative splicing.
Specificity
Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Epitope: N-terminus
Anti-Tropomyosin-1, clone 7F8B8, Cat. No. MABT1365, is a rat monoclonal antibody that detects Tropomyosin-1 and has been tested for use in Immunocytochemistry and Western Blotting.
Research Category
Cell Structure
Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Quality
Evaluated by Western Blotting in mouse embryonic fibroblast (MEF) lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin-1 in mouse embryonic fibroblast (MEF) lysate.
Target description
~30 kDa observed; 28.38 kDa for isoform 5. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	7F8B8, monoclonal
species reactivity	human, mouse
packaging	antibody small pack of 25 µL
application(s)	immunocytochemistry: suitable","western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_000357.3,
UniProt accession no.	P09493,