Anti-TRIO

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABN1452

ABN1452-25UG

Triple functional domain protein (UniProt: O75962; also known as EC: 2.7.11.1, PTPRF-interacting protein, TRIO) is encoded by the TRIO gene (Gene ID: 7204) in human. TRIO is a complex protein that is widely expressed in human tissue, with highest levels reported in heart, skeletal muscle, and brain. Five isoforms of TRIO have been reported that are produced by alternative splicing. It contains three catalytic domains, two tandem Dbl Homology (DH) and pleckstrin homology (PH) domains, and a serine/Threonine kinase domain. The DH-PH module is involved in guanine nucleotide exchange and the activation of the small GTPases of the Rho family. The DH1-PH1 domain (GEF1) is reported to activate the Rho G and Rac1, whereas DH2-PH2 (GEF2) is involved in the activation of RhoA. TRIO is involved in coordinating actin remodeling, which is necessary for cell migration and growth. In developing hippocampal neurons it limits dendrite formation, without affecting the establishment of axon polarity. After dendrites are formed, it is reported to be involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors at CA1 excitatory synapses. Mutations in TRIO gene have been linked to mental retardation and microcephaly. (Ref.: Debant, A. et al. (1996). Proc. Natl. Acad. Sci, USA. 93(11); 5466-71; Bellanger, JM. Et al. (2000). Nat. Cell Biol. 2(12); 888-92).
Specificity
This rabbit polyclonal antibody detects human Triple functional domain protein (TRIO). It targets an epitope within 17 amino acids from the C-terminal half. The immunogen sequence is conserved in all 5 isoforms.
KLH-conjugated liner peptide corresponding to 17 amino acids from the C-terminal half of human Triple functional domain protein.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected TRIO in human cerebral cortex, human pancreas, and human testis tissue.
Anti-TRIO Antibody, Cat. No. ABN1452, is a highly specific rabbit polyclonal antibody that targets Triple functional domain protein (TRIO) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRIO in 10 µg of HEK293 cell lysate.
Target description
~300 kDa observed; 346.90 kDa calculalted. Uncharacterized bands may be observed in some lysate(s).
Concentration: Please refer to lot specific datasheet.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_009049.2,
UniProt accession no.	O75962,
shipped in	ambient
Gene Information	human ... TRIO(7204)

Flash Point F	does not flash
Flash Point C	does not flash