Anti-TMC1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABN1649

ABN1649-25UL

Transmembrane channel-like protein 1 (UniProt: Q8R4P5; also known as Beethoven protein, Deafness protein, Transmembrane cochlear-expressed protein 1, TMC1) is encoded by the Tmc1 (also known as Bth, dn) gene (Gene ID: 13409) in murine species. TMC1 is a multi-pass membrane protein that forms ion channel required for the normal function of cochlear hair cells. It is detected in cochlear inner and outer hair cells and in neurosensory epithelia of the vestibular end organs. Its expression is also reported in cortex, cerebellum, eye, colon, ovary, and testis. It is expressed at low levels in temporal bone from embryonic day 14 to day 1 after birth in mice and increases significantly (8 to 16 fold) at day 5, 10 and 20 and then continues to be expressed up to day 90. Two isoforms of TMC1 have been reported in mice that are produced by alternative splicing. Mutations in Tmc1 gene can cause hearing loss in humans and mice. Heterozygotes show progressive hair-cell degeneration from day 20 onwards in mice, leading to severe depletion of inner hair cells and scattered loss of outer hair cells, and progressive loss of the Preyer reflex from around day 30. Homozygotes show almost complete degeneration of inner hair cells, and little or no Preyer reflex at any age.
Specificity
This rabbit polyclonal antibody detects Transmembrane channel-like protein 1 in murine retina. It targets an epitope within 20 amino acids from the N-terminal region.
Epitope: cytoplasmic domain
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal cytoplasmic domain of murine Transmembrane channel-like protein 1.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMC1 in COS-7 cells.
Immunofluorescence Analysis: A representative lot detected TMC1 in P9 mouse outer hair cells (OHCs) (Kurima, K., et. al. (2015). Cell Rep. 12(10):1606-17).
Western Blotting Analysis: A representative lot detected TMC1 in COS-7 cells (Labay, V., et. al. (2010). Biochemistry. 49(39):8592-8).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TMC1 in HEK293 cells transiently transfected with Fugene and TMCx-cherry cDNAs (Courtesy of Dr. Angela Ballesteros).
Immunofluorescence Analysis: A representative lot detected TMC1 in COS-7 transfected cells expressing TMC1-GFP (Courtesy of Dr. Angela Ballesteros).
Anti-TMC1, Cat. No. ABN1649, is a highly specific rabbit polyclonal antibody that targets Transmembrane channel-like protein 1 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Research Category
Neuroscience
Quality
Evaluated by Western Blotting in mouse retinal tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TMC1 in 10 µg of mouse retinal tissue lysate.
Target description
~90 kDa observed; 87.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	monkey, mouse
packaging	antibody small pack of 25 µL
application(s)	immunocytochemistry: suitable","immunofluorescence: suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_083229,
UniProt accession no.	Q8R4P5,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable