Anti-TKS5 Antibody, clone 9H2.1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABT337

MABT337-25UG

SH3 and PX domain-containing protein 2A (UniProt: Q5TCZ1; also known as Adapter protein TKS5, Five SH3 domain-containing protein, SH3 multiple domains protein 1, Tyrosine kinase substrate with five SH3 domains) is encoded by the SH3PXD2A (also known as FISH, KIAA0418, SH3MD1, TKS5) gene (Gene ID: 9644) in human. Adapter protein TKS5 is a scaffolding protein that is required for podosome formation, degradation of the extracellular matrix, and for invasion in some cancer cells. Three isoforms of TKS5 have been described that are produced by alternative splicing. TKS5 is a cytoplasmic protein in normal cells, but it localizes to podosomes in SRC-transformed cells. It is found in several cancer cell lines, particularly invasive breast carcinomas and melanomas. TKS5 contains one PX (phx homology) domain and five SH3 domains. The PX domain is required for podosome localization because of its ability to bind phosphoinosiltols, such as PtdIns3P and PtdIns(3,4)P2 and to lesser extent PtdIns5P and PtdIns(3,5)P2. Its binding with ADAM12, ADAM15 and ADAM19 is mediated by the fifth SH3 domain. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region.
Specificity
Clone 9H2.1 detects SH3 and PX domain-containing protein 2A (adapter protein TKS5) in human cells. It targets and epitope with in 91 amino acids crom the C-terminal half.
GST/His-tagged recombinant fragment corresponding to 91 amino acids from the C-terminal half of human SH3 and PX domain-containing protein 2A (Adapter protein TKS5).
This mouse monoclonal Anti-TKS5, clone 9H2.1, Cat. No. MABT337, is a mouse monoclonal antibody that detects SH3 and PX domain-containing protein 2A (TKS5) and has been tested for use in Western Blotting.
Research Category
Cell Structure
Quality
Evaluated by Western Blotting in SCC61, SK-MEL28, and T47D cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected TKS5 in 10 µg of SCC61, SK-MEL28, and T47D cell lysates.
Target description
~125 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	9H2.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NP_055446,
UniProt accession no.	Q5TCZ1,
shipped in	ambient
Gene Information	human ... SH3PXD2A(9644)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable