Anti-TIMP-4 Antibody, clone 9:4-7

eCl@ss:

32160702

Кат. номер

MABC1142-25UL

MABC1142-100UL

Tissue inhibitor of metalloproteinases 4 (UniProt: Q99727; also known as Metalloproteinase inhibitor 4, TIMP-4) is encoded by the TIMP4 gene (Gene ID: 7079) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins which are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMP-4 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has two MMP-binding regions (aa 30-33 and 99-100). TIMP-4 expressed is observed in heart, brain, pancreas, kidney, colon, testes, and adipose tissue. However, elevated levels of TIMP-4 have been reported in various cancers tissues, including breast, ovary, prostate, colon and kidney and are associated with poor survival prognosis. Hence, it can serve as a useful marker for disease progression. TIMP-4 is shown to complex MMPs and irreversibly inactivates them by binding to their catalytic zinc cofactor. This clone (9:4 7) is specific for human TIMP-4 and does not exhibit any cross-reactivity to other TIMPs. (Ref.: Gomez, DE., et al. (1997). Eur. J. Cell. Biol. 74(2); 111-122; Donover, PS et AL (2010). J. Cell. Biochem. 110(5); 1255-1261).
Specificity
Clone 9:4-7 specifically detects human TIMP-4.
GST-tagged full-length recombinant human TIMP-4.
Anti-TIMP-4, clone 9:4-7, Cat. No. MABC1142, is a mouse monoclonal antibody that detects Tissue Inhibitor of Matrix Metalloproteinase 4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected TIMP-4 in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A representative lot detected TIMP-4 in Immunohistochemistry applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).
ELISA Analysis: A representative lot detected TIMP-4 in ELISA applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).
Western Blotting Analysis: A representative lot detected TIMP-4 in Western Blotting applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).
Research Category
Apoptosis & Cancer
Quality
Evaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected TIMP-4 in human breast cancer tissue sections.
Target description
25.50 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	4-7, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	ELISA: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_003247.1,
UniProt accession no.	Q99727,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable