- Производитель:
- Sigma-Aldrich
Кат. номер |
MABF2657-25UL |
MABF2657-100UL |
Specificity
Clone 1G9 is a mouse monoclonal antibody that detects murine T-cell immunoreceptor with Ig and ITIM domains (TIGIT).
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected T-cell immunoreceptor with Ig and ITIM domains (TIGIT) s in RAW264.7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIGIT in mouse thymus tissue lysate.
ELISA Analysis: A representative lot detected TIGIT in ELISA applications (Joller, N., et al. (2011). J Immunol. 186(3):1388-42).
Flow Cytometry Analysis: A representative lot detected TIGIT in Flow Cytometry applications (Joller, N., et al. (2011). J Immunol. 186(3):1388-42).
Inhibition Assay: A representative lot of this antibody fully blocked CD155 binding to TIGIT. (Dixon, K.O., et al. (2018). J Immunol. 200(8):3000-3007).
Flow Cytometry Analysis: 1 µg from a representative lot detected TIGIT in one million Raw264.7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TIGIT, clone 1G9, Cat. No. MABF2657, is a mouse monoclonal antibody that detects of T-cell immunoreceptor with Ig and ITIM domains (TIGIT) and is tested in ELISA, Flow Cytometry, Inhibition Assay, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Level | 200 |
biological source | mouse |
antibody form | purified antibody |
antibody product type | primary antibodies |
clone | 1G9, monoclonal |
mol wt | calculated mol wt 26.96 kDa","observed mol wt ~31 kDa |
purified by | using protein G |
species reactivity | mouse |
packaging | antibody small pack of 100 µL |
application(s) | ELISA: suitable","flow cytometry: suitable","western blot: suitable |
isotype | IgG1 |
epitope sequence | Unknown |
conjugate | unconjugated |
UniProt accession no. | P86176, |
shipped in | dry ice |
Gene Information | mouse ... Tigit(100043314) |
WGK Germany | WGK 2 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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