Anti-Tetranectin/CLEC3B Antibody, clone mAb10

Кат. номер

MABT1444-25UG

MABT1444-100UG

Tetranectin (UniProt: P05452; also known as TN, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein) is encoded by the CLEC3B (also known as TNA) gene (Gene ID: 7123) in human. Tetranectin is a tetrameric plasminogen-binding protein that is synthesized with a signal peptide (aa 1-21), which is subsequently removed in the mature form that is composed of three identical, noncovalently linked subunits each of 181 amino acids. The polypeptide consists of a short a-helical segment (aa 47-72) at the amino terminus that is involved in trimerization, followed by a C-type lectin domain (aa 77-198). It is present in both serum and the extracellular matrix and is expressed most abundantly in the lung. The blood concentrations of tetranectin in healthy adults are about 10-12 mg/mL. However, in septic subjects its levels are significantly reduced and supplementation with exogenous tetranectin confers dose-dependent protection against lethal endotoxemia and sepsis. Its levels are shown to be significantly reduced in patients with cardiac failure. Hence, it is also used as a biomarker for risk of heart failure. (Ref.: Chen, W., et al. (2020). Sci. Transl. Med. 12(539); eaaz3833; McDonald, K., et al. (2020). Sci. Rep. 10; 7507).
Specificity
Clone mAb10 is a mouse monoclonal antibody that detects Tetranectin. It targets an epitope within 24 amino acids from the C-terminal half.
Purified human Tetranectin.
Anti-Tetranectin/CLEC3B, clone mAb10, Cat. No. MABT1444, is a mouse monoclonal antibody that detects Tetranectin and is tested for use in Dot Blot, ELISA, and Western Blotting.
Quality Conrol Testing
Evaluated by Western Blotting in Human lung tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tetranectin/CLEC3B in Human lung tissue lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected Tetranectin/CLEC3B in Western Blotting applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).
Dot Blot: A representative lot detected Tetranectin/CLEC3B in Dot Blot applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).
ELISA Analysis: A representative lot detected Tetranectin/CLEC3B in ELISA applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	mAb10, monoclonal
mol wt	calculated mol wt 22.54 kDa","observed mol wt ~25 kDa
purified by	using protein G
species reactivity	human
packaging	antibody small pack of 100 µg
application(s)	ELISA: suitable","dot blot: suitable","western blot: suitable
isotype	IgG2b
epitope sequence	C-terminal half
conjugate	unconjugated
Protein ID accession no.	NP_003269,
UniProt accession no.	P05452,
shipped in	2-8°C
Gene Information	human ... CLEC3B(7123)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable