Anti-TCR V7 Antibody, clone F2.67

Кат. номер

MABF2676-25UL

MABF2676-100UL

T cell receptor gamma, variable 7 (UniProt: A0A0A6YYE8; also known as TCR Vg7) is encoded by the Trgv7 (also known as Tcrg-V7) gene (Gene ID: 21641) in murine species. The T-cell receptor (TCR) is a protein complex found on the surface of T cells that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. The TCR is a heterodimer of a and b chain and over 95% of T cells express these two chains and only about 5% of T cells carry g and d chains, which are the first T lymphocytes of innate immunity to arise in ontogeny. The diversity of TCR arises from genetic recombination of the DNA-encoded segments in individual somatic T cells by somatic V(D)J recombination using RAG1 and RAG2 recombinases. This recombination process is reported to be unique to lymphocytes during the early stages of their development in primary lymphoid organs. The generation of the TCR g chain involves VJ recombination. There are seven Vg gene segments have been described in the TCRg chain loci of mice. TCR Vg7 is localized on the external side of plasma membrane. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce the mature form. In most inbred strains of mice, T cells expressing Vg7- or Vg1- encoded TCRs make up 90% of intestinal intraepithelial lymphocytes. These cells are reported to be essential to limit parasite invasion and their dissemination from the intestinal mucosa. (Ref.: Li, C., et al. (2012). Cell. Immunol. 279(2); 151-159; Dalton, JE., et al. (2006). Gatroenterol.131(3); 818-829; Pereira, ,P., et al. (2000). Eur. J. Immunol. 30(7); 1988-1997).
Specificity
Clone F2.67 is a mouse monoclonal antibody generated in TCR delta knockout mice. It detects murine T cell receptor g, variable 7.
Mouse hybridoma expressing anti V 7V 5 TCR.
Quality Control Testing
Evaluated by Flow Cytometry in Balb/c mouse thymocytes.
Flow Cytometry Analysis: 1.0 µg of this antibody detected T-cell Vg 7 in one million Balb/c mouse thymocytes.
Tested Applications
Flow Cytometry Analysis: A representative lot detected TCR Vg7 in Flow Cytometry applications (Pereira, P., et al. (2000). Eur J Immunol.;30(7):1988-97; Dalton, J.E., et al. (2006). Gastroenterology.;131(3):818-29; Newton, D.J., et al. (2006). Infect Immun.;74(2):1097-105; Hamada, S. et al. (2008). Immunology.;125(2):170-7; Li, C., et al. (2012). Cell Immunol.;279(2):151-9; Walker, C.R., et al. (2013). PLoS One.;8(12):e84553).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TCR Vg7, clone F2.67, Cat. No. MABF2676, is a mouse monoclonal antibody that detects T cell receptor g, variable 7 and is tested for use in Flow Cytometry.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	F2.67, monoclonal
mol wt	calculated mol wt 13.53 kDa
purified by	using protein G
species reactivity	mouse
packaging	antibody small pack of 100 µL
application(s)	flow cytometry: suitable
isotype	IgG2a
epitope sequence	Unknown
conjugate	unconjugated
UniProt accession no.	A0A0A6YYE8,
shipped in	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable