Anti-STRN3

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS2099

ABS2099-25UG

Striatin-3 (UniProt: Q13033; also known as Cell cycle autoantigen SG2NA, S/G2 antigen) is encoded by the STRN3 (also known as GS2NA, SG2NA) gene (Gene ID: 29966) in human. Striatin-3 is a calmodulin-binding protein that bonds calmodulin a calcium dependent manner and may function as scaffolding or signaling protein. Two isoforms of Striatin-3 have been reported that are produced by alternative splicing. Its calmoduln-binding region is located within amino acids 166-183. It also contains a caveolin-binding region (aa 71-79), six WD repeats, and a coiled coil region (aa 77-136). The coiled coil domain and the PP2A subunit can form a stable core complex with a 2:2 stoichiometry. The coiled coil domain of striatin-3 is a novel type of PP2A regulatory subunit that functions as a scaffold for the assembly of the STRIPAK complex. PP2A and certain kinases, such as germinal central kinase III (GCKIII) interact with striatin to form striatin-interacting phosphatase and kinase (STRIPAK) complex that are known to regulate multiple cellular events. Wild-type striatin-3 is shown to strongly suppress apoptosis in Jurkat cells induced by the GCKIII kinase MST3. However, mutant forms of striatin-3 fail to exhibit this effect. (Ref.: Lu, Q., et al. (2004). Proc. Natl. Acad. Sci. USA 101(49); 17126-17131; Chen, C., et al. (2014). J. Biol. Chem. 289(14); 9651-61).
Specificity
This rabbit polyclonal antibody detect Striatin-3 in human and murine cells. It targets an epitope within 17 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Striatin-3. The immunogen sequence is conserved in both alpha and beta isoforms.
Epitope: unknown
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STRN3 in HeLa cells.
Research Category
Signaling
Anti-STRN3, Cat. No. ABS2099, is a rabbit polyclonal antibody that detects Striatin-3 and has been tested for use in Immunocytochemistry and Western Blotting.
Quality
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected STRN3 in 10 µg of NIH3T3 cell lysate.
Target description
~87 kDa observed; 87.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human, mouse
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_001077362,
UniProt accession no.	Q13033,
shipped in	ambient
Gene Information	human ... STRN3(29966)

Flash Point F	does not flash
Flash Point C	does not flash