Anti-Strep-Tag Antibody, clone C23.21

Кат. номер

MAC143-25UL

MAC143-100UL

Strep-Tag is a short amino acid-peptide sequence that exhibits specific binding to streptavidin. It is shown to reversibly occupy the same pocket where biotin is normally complexed. Hence, it is highly desirable for applications involving where efficient purification of fusion proteins. Use of this tag allows for easier detection of fusion proteins in various immunochemical assays. Strep-Tag is shown to be resistant to the action of proteases and can also be used in the presence of mild detergents. Clone C23.21 recognizes fusion proteins with Strep-Tag peptide sequence WSHPQFEK with picomolar affinity. It recognizes both the single and the double Strep-Tag sequence.
Specificity
Clone C23.21 is a mouse monoclonal antibody that detects Strep-Tagged proteins. It binds to WSHPQFEQ sequence with picomolar affinity.
Recombinant ectodomain of glycoprotein E2 of GB virus (GBV-B), containing a double Strep-Tag (two Strep-Tags separated by a linker peptide)
Anti-Strep-Tag, clone C23.21, Cat. No. MAC143, is a mouse monoclonal antibody that detects Strep-Tagged proteins and is tested for use in Immunohistochemistry, Surface plasmon resonance, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting with Strep-Tagged VHH 9.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Strep-Tagged VHH9.
Tested Applications
Surface plasmon resonance: A representative lot detected Strep-Tag in Surface plasmon resonance application. (Meola, A., et al. (2015). J Virol. 89(4):2170-81).
Western Blotting Analysis: A representative lot detected Strep-Tag in human brain extract and GFAP in Western Blotting applications. (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Immunohistochemistry Applications: A representative lot detected Strep-Tag in mouse brain tissue sections in Immunohistochemistry applications (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	C23.21, monoclonal
mol wt	observed mol wt ~17 kDa
purified by	using protein G
species reactivity	all
packaging	antibody small pack of 100 µL
application(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG1
epitope sequence	Unknown
conjugate	unconjugated
UniProt accession no.	N/A,
shipped in	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable