Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480

eCl@ss:

32160702

Кат. номер

MAB4301

MAB4301-25UG

MAB4301-100UG

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Specificity
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
F9 tetracarcinoma stem cells (X-irradiated).
Research Sub Category
Pluripotent & Early Differentiation
Neural Stem Cells
Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Immunohistochemistry:
A previous lot of this antibody was tested on IH.
Immunoprecipitation:
A previous lot of this antibody was tested on IP.
Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.
Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.
Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Quality
Tested
Target description
220 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
Ammonium Sulfate Precipitation
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
P19 mouse embryonic carcinoma cells
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	saturated ammonium sulfate (SAS) precipitated
antibody product type	primary antibodies
clone	MC-480, monoclonal
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µg
Торговая марка	Chemicon®
application(s)	flow cytometry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable
input	sample type: human embryonic stem cell(s) sample type: mouse embryonic stem cell(s) sample type induced pluripotent stem cell(s) sample type neural stem cell(s)
isotype	IgM
NCBI accession no.	NM_002033.2,
UniProt accession no.	P22083,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... FUT4(2526)