Anti-SREBP-1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS1508

ABS1508-25UG

Sterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human. SREBP-1 is a multi-pass membrane protein that acts as at transcriptional activator required for lipid homeostasis. Six isoforms of SREBP-1 have been described that are produced by alternative splicing. SREBP-1 is expressed in a wide variety of tissues and is most abundant in liver and adrenal gland. In fetal lung and liver it is expressed in high levels. SREBP-1A predominates in hepatoma cell lines while isoform SREBP-1C is predominantly found in liver, adrenal gland, and ovary. Both isoforms IA and IC is found in kidney, brain, white fat, and muscle. SREBP-1 regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. It binds to the sterol regulatory element 1 (SRE-1) (5-ATCACCCCAC-3) and has dual sequence specificity binding to both an E-box motif (5-ATCACGTGA-3) and to SRE-1 (5-ATCACCCCAC-3). SREBP-1 has two cytoplasmic domains (aa 1-487 and 567-1147; two helical domains (aa 488-508 and 548-568), and a luminal domain (aa 509-547). In the absence of sterols, it moves from the endoplasmic reticulum to the Golgi. At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane.
Specificity
This rabbit polyclonal antibody detects Sterol regulatory element-binding protein 1 in human.
KLH-conjugated linenar peptide corresponding to 14 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1. The Immunogen sequence is conserved in SREBP-1A, B, and C and in isoform 4.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SREBP-1 in human adrenal gland and human kidney tissues.
Research Category
Signaling
Anti-SREBP-1, Cat. No. ABS1508, is a rabbit polyclonal antibdody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in DU145 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected SREBP-1 in 10 µg of DU145 cell lysate.
Target description
~120 kDa observed; 121.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
UniProt accession no.	P36956,
shipped in	ambient

Flash Point F	does not flash
Flash Point C	does not flash